Neutralization of proANP (1-30) exacerbates hypertension in the spontaneously hypertensive rat.
Neutralization of proANP (1-30) exacerbates hypertension in the spontaneously hypertensive rat.
复制标题
proANP (1-30) 的中和会加剧自发性高血压大鼠的高血压。
DOI：
10.1046/j.1440-1681.2003.03888.x
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发表时间：
2003
影响因子：
2.9
通讯作者：
Nazian,StanleyJ
中科院分区：
文献类型：
--
作者：
Dietz,JohnR;Vesely,DavidL;Gower,WilliamR;Landon,CarolS;Lee,SookJeong;Nazian,StanleyJ
1.本研究的目的是确定proANP（1-30）在动脉压调节中的作用。假设阻断内源性proANP（1-30）会加重易感动物模型中的高血压。将戊巴比妥麻醉的自发性高血压大鼠（SHR）静脉注射1.2 mL含有特异抗大鼠proANP抗体（1-30）的兔血清（SHR‐AB组，n= 7）或等量正常兔血清作为对照（SHR‐NRS组，n= 5）。在1小时的平衡期和2个30分钟的基线期后，大鼠在Krebs溶液中加入3ml 6%白蛋白进行体积扩张，并观察另外3小时，以确定抗proANP对动脉压的影响。两组动脉压均随容量扩张而升高，但在整个容量扩张期间，抗proANP SHR组的动脉压明显高于SHR - NRS组。抗proANP SHR组与NRS - SHR组在研究开始150分钟时最大差异为21 mmHg（分别为183±5vs162±3mmhg， P< 0.005.5）。这些结果提示proANP（1-30）在高血压SHR模型中具有保护作用。
1. The aim of the present studies was to determine the role of proANP (1–30) in the regulation of arterial pressure. It was hypothesized that blocking endogenous proANP (1–30) would exacerbate the hypertension in susceptible animal models.2. Pentobarbital‐anaesthetized spontaneously hypertensive rats (SHR) were pretreated i.v. with 1.2 mL rabbit serum containing an antibody directed specifically against rat proANP (1–30) (SHR‐AB group;n= 7) or an equal volume of normal rabbit serum as a control (SHR‐NRS group;n= 5).3. Following a 1 h equilibration period and two 30 min baseline periods, rats were volume expanded with 3 mL of 6% albumin in Krebs' solution and observed for an additional 3 h to determine the effects of the anti‐proANP on arterial pressure.4. Arterial pressure increased in both groups compared with their own baselines with volume expansion, but was significantly greater in the anti‐proANP SHR group compared with the SHR‐NRS group throughout the volume expansion period. A maximum difference of 21 mmHg between the anti‐proANP SHR group and the NRS‐SHR group was observed at 150 min of the study (183 ± 5vs162 ± 3 mmHg, respectively;P< 0.005.5. These results suggest a protective role for proANP (1–30) in the SHR model of hypertension.