Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells
Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells
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DOI：
10.1016/j.bbrc.2005.12.050
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发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Yokochi, T
中科院分区：
文献类型：
--
作者：
Islam, S;Hassan, F;Yokochi, T
本文研究了脂多糖（LPS）对内质网（ER）应激剂诱导的RAW 264.7细胞死亡的影响。LPS可阻止布雷菲德菌素A引起的细胞死亡，但不能阻止毒胡萝卜素和衣霉素引起的细胞死亡。CpG DNA以及LPS阻止布雷菲德菌素A诱导的细胞死亡，而肿瘤坏死因子-α或干扰素-γ没有。Brefeldin A诱导的细胞死亡是通过凋亡细胞死亡介导的，并且它被LPS显著抑制。LPS可抑制内质网应激相关的半胱天冬酶（如半胱天冬酶1、3和4）的活化。LPS阻止了布雷菲德菌素A诱导的RAW 264.7细胞的形态学变化。此外，LPS阻止布雷菲德菌素A诱导的高尔基体分散。提示LPS可减轻Brefeldin A对ER/Golgi复合体的应激，抑制细胞凋亡。LPS对布雷菲德菌素A诱导的细胞凋亡的预防作用进行了讨论。(c)2005年爱思唯尔公司All rights reserved.
The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-alpha or interferon-gamma did not. Brefeldin A-induced cell death was mediated with apoptotic cell death and it was significantly inhibited by LPS. LPS abolished the activation of ER stress-related caspases, such as caspases 1, 3, and 4. LPS prevented brefeldin A-induced morphological changes in RAW 264.7 cells. Further, LPS prevented brefeldin A-induced Golgi dispersion. Therefore, LPS was suggested to diminish the stress of ER/Golgi complexes induced by brefeldin A and inhibit apoptosis. The preventive action of LPS on brefeldin A-induced apoptosis is discussed. (c) 2005 Elsevier Inc. All rights reserved.