In vitro susceptibilities to fluoroquinolones in current and archived Mycoplasma gallisepticum and Mycoplasma synoviae isolates from meat-type turkeys
In vitro susceptibilities to fluoroquinolones in current and archived Mycoplasma gallisepticum and Mycoplasma synoviae isolates from meat-type turkeys
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DOI：
10.1016/j.vetmic.2008.04.006
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发表时间：
2008-10-15
影响因子：
3.3
通讯作者：
Levisohn, Sharon
中科院分区：
文献类型：
--
作者：
Gerchman, Irina;Lysnyansky, Inna;Levisohn, Sharon
监测禽致病性支原体野外分离株对抗生素的敏感性对合理选择治疗方案具有重要意义。本研究比较了最近（2005-2006年）从肉型火鸡群中分离出的鸡脓支原体和滑膜支原体与已存档的（1997-2003年）分离株和参考菌株对恩诺沙星和双氟沙星的体外敏感性。通过微试、琼脂稀释和商业测试测定的最低抑制浓度（MIC）值的比较显示出良好的一致性，但强调了标准化测试方法的必要性。值得注意的是，在0.002 ~ 0.094 μ g/ml范围内测定恩诺沙星的MIC方便、准确，但MIC值>= 1.0 μ g/ml时，无法确定对鸡链球菌和滑膜链球菌的抑制终点。在存档菌株中检测到对这两种氟喹诺酮类药物的敏感性下降，但在最近分离的菌株中发现的程度更大，其中大多数菌株的mic高于ncls对这两种抗生素的敏感性断点(
Monitoring of susceptibility to antibiotics in field isolates of pathogenic avian mycoplasmas is important for appropriate choice of treatment. Our study compared in vitro susceptibility to enrofloxacin and difloxacin in recent (2005-2006) isolates of Mycoplasma gallisepticum and Mycoplasma synoviae from meat-type turkey flocks with archived (1997-2003) isolates and reference strains. Comparison of minimal inhibitory concentration (MIC) values determined by microtest, agar dilution and commercial Etest showed good agreement, but underscored the need for standardized methods for testing. Notably, while the commercial Etest was convenient and accurate for determining MICs for enrofloxacin in the range 0.002-0.094 mu g/ml, the endpoint of inhibition for M. gallisepticum and M. synoviae strains with MIC values >= 1.0 mu g/ml could not be determined. A decrease in susceptibility to both fluoroquinolones was detected in archived strains but to a greater degree in recent isolates, most of which had MICs above the NCCLS susceptibility breakpoint for these antibiotics (