Identificaiton of a clubroot resistance locus conferring resistance to a Plasmodiophora brassicae classified into pathotype group 3 in Chinese cabbage (Brassica rapa L.).
Identificaiton of a clubroot resistance locus conferring resistance to a Plasmodiophora brassicae classified into pathotype group 3 in Chinese cabbage (Brassica rapa L.).
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DOI：
10.1270/jsbbs.62.282
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发表时间：
2012-09
期刊：
影响因子：
2.4
通讯作者：
Matsumoto S
中科院分区：
文献类型：
--
作者：
Kato T;Hatakeyama K;Fukino N;Matsumoto S
在大白菜（Brassica rapa）中，根肿病抗性（CR）基因Crr 1和Crr 2对温和的芸苔根肿菌分离物Ano-01和更强的分离物和歌山-01有效，但对分类为致病型组3的分离物No. 14无效。抗根肿病的F_1代品种‘秋里生’对14号菌株表现出抗性。为了提高抗性的持久性，我们试图鉴定'Akiriso'中的CR位点。CR在'Akiriso'分离作为一个单一的显性基因，并与几个分子标记，也与CRb，CR基因座从品种'CR Shinki'。我们在CRb周围开发了额外的标记，并在'Akiriso'和'CR Shinki'中构建了该区域的部分遗传图谱。标记在两个品种遗传图谱上的位置和顺序非常相似。两个品种的F2群体对14号分离物的抗性分离率也非常相似。这些结果表明，'Akiriso'的CR位点是CRb或紧密连锁的位点。本研究所开发的标记与大白菜CRb基因的连锁关系较以往报道的标记更为紧密，为大白菜CRb基因的分子标记辅助选择提供了依据。
In Chinese cabbage (Brassica rapa), the clubroot resistance (CR) genes Crr1 and Crr2 are effective against the mild Plasmodiophora brassicae isolate Ano-01 and the more virulent isolate Wakayama-01, but not against isolate No. 14, classified into pathotype group 3. ‘Akiriso’, a clubroot-resistant F1 cultivar, showed resistance to isolate No. 14. To increase the durability of resistance, we attempted to identify the CR locus in ‘Akiriso’. CR in ‘Akiriso’ segregated as a single dominant gene and was linked to several molecular markers that were also linked to CRb, a CR locus from cultivar ‘CR Shinki’. We developed additional markers around CRb and constructed partial genetic maps of this region in ‘Akiriso’ and ‘CR Shinki’. The positions and order of markers in the genetic maps of the two cultivars were very similar. The segregation ratios for resistance to isolate No. 14 in F2 populations derived from each of the two cultivars were also very similar. These results suggest that the CR locus in ‘Akiriso’ is CRb or a tightly linked locus. The newly developed markers in this study were more closely linked to CRb than previously reported markers and will be useful for marker-assisted selection of CRb in Chinese cabbage breeding.