Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles
Nanomaterial-Based Fluorescent DNA Analysis: A Comparative Study of the Quenching Effects of Graphene Oxide, Carbon Nanotubes, and Gold Nanoparticles
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基于纳米材料的荧光 DNA 分析：氧化石墨烯、碳纳米管和金纳米颗粒猝灭效应的比较研究
DOI：
10.1002/adfm.201203816
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发表时间：
2013-09-06
影响因子：
19
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Li, Fan;Pei, Hao;Fan, Chunhai
各种纳米材料对范围广泛的荧光团都表现出了极高的猝灭能力。最近，人们对开发新的荧光DNA分析工具产生了浓厚的兴趣，这些工具基于这一性质，并通过利用这些纳米级超级猝灭剂与单链或双链形式的DNA分子之间的相互作用来进行分析。在这里，使用一系列不同维度的纳米材料，即金纳米颗粒(AuNPs，0D)、碳纳米管(CNTs，1D)和氧化石墨烯(GO，2D)，对纳米振动效应进行了比较研究。对其猝灭效率、动力学、分化能力以及浓度、离子强度等影响因素进行了研究。有趣的是，GO在猝灭效率和动力学方面都表现出比其他两种材料更好的猝灭能力。因此，以简单的混合检测格式设计的基于GO的荧光传感器可以检测低至0.2 nm的DNA浓度，这比碳纳米管或AuNPs好一个数量级。这种传感器也可以比基于碳纳米管或基于AuNPs的传感器更好地区分单碱基错配。这项研究为更好地选择用于生物分析的纳米材料和精心设计用于体外诊断和体内生物成像的生物传感器铺平了道路。
A variety of nanomaterials have shown extraordinarily high quenching ability toward a broad range of fluorophores. Recently, there has been intense interest in developing new tools for fluorescent DNA analysis in solution or inside the cell based on this property, and by exploiting interactions between these nanoscale superquenchers and DNA molecules in the single-stranded (ss-) or double-stranded (ds-) forms. Here, a comparative study on the nanoqueching effects is performed by using a series of nanomaterials with different dimensions, i.e., gold nanoparticles (AuNPs, 0D), carbon nanotubes (CNTs, 1D), and graphene oxide (GO, 2D). The quenching efficiency, kinetics, differentiation ability, and influencing factors such as concentration and ionic strength are studied. Interestingly, GO exhibits superior quenching abilities to the other two materials in both the quenching efficiency and kinetics. As a result, a GO-based fluorescent sensor, designed in a simple mix-and-detect format, can detect concentrations of DNA as low as 0.2 nM, which is better than either CNTs or AuNPs by an order of magnitude. This sensor can also differentiate single-base mismatches much better than either CNTs- or AuNPs- based sensors. This study paves the way to better choice of nanomaterials for bioanalysis and elaborate design of biosensors for both in vitro diagnosis and in vivo bioimaging.