OCTACALCIUM PHOSPHATE CARBOXYLATES .1. PREPARATION AND IDENTIFICATION
OCTACALCIUM PHOSPHATE CARBOXYLATES .1. PREPARATION AND IDENTIFICATION
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DOI：
10.1021/cm00034a007
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发表时间：
1993-10-01
影响因子：
8.6
通讯作者：
BROWN, WE
中科院分区：
文献类型：
--
作者：
MARKOVIC, M;FOWLER, BO;BROWN, WE
通过在19种羧酸铵（单羧酸盐;饱和、不饱和、羟基、酮基和氨基二羧酸盐;和三羧酸盐）的溶液中转化α-Ca 3（PO 4）2，研究了磷酸八钙羧酸盐Ca 8（HPO 4）m（羧酸盐）n（PO 4）4·yH 2 O的形成。通过X射线衍射和红外和拉曼光谱确定所形成的各种固相，这取决于初始pH和转化时间。形成并鉴定了含有结构上结合的丙二酸、琥珀酸、己二酸、辛二酸、癸二酸、富马酸、苹果酸和柠檬酸离子的磷酸八钙羧酸盐。磷酸八钙羧酸盐也形成丙酮酸和α-酮戊二酸溶液，但不确定的羧酸根离子结构。所有这些鉴定的化合物在结构上类似于磷酸八钙，Ca 8（HPO 4）2（PO 4）4·5 H2O，但具有扩展的a轴晶胞尺寸，其通常随着羧酸根离子中碳原子数目的增加而增加。在这些化合物中，特别重要的是含有作为克雷布斯循环中的中间体存在的羧酸盐的那些。这些磷酸八钙的可能沉淀在线粒体和钙化组织形成的前体可能发挥的作用进行了讨论。
The formation of octacalcium phosphate carboxylates, Ca8(HPO4)m(carboxylate)n(PO4)4.yH2O, by conversion of alpha-Ca3(PO4)2 in solutions of 19 ammonium carboxylates (monocarboxylates; saturated, unsaturated, hydroxy, keto, and amino dicarboxylates; and tricarboxylates) was investigated. The various solid phases formed, depending on initial pH's and conversion times, were determined by X-ray diffraction and infrared and Raman spectroscopy. Octacalcium phosphate carboxylates containing structurally incorporated malonate, succinate, adipate, suberate, sebacate, fumarate, malate, and citrate ions were formed and identified. Octacalcium phosphate carboxylates were also formed from pyruvate and alpha-ketoglutarate solutions but with uncertain carboxylate ion structures. All of these identified compounds are structurally similar to octacalcium phosphate, Ca8(HPO4)2(PO4)4-5H2O, but have expanded a-axis unit-cell dimensions that generally increased with increasing number of carbon atoms in the carboxylate ion. Among these compounds, of special importance are those containing carboxylates that are present as intermediates in the Krebs cycle. The possible precipitation of these octacalcium phosphate carboxylates in mitochondria and their possible role as precursors in calcified tissue formation are discussed.