Long-term toxicity of reduced graphene oxide nanosheets: Effects on female mouse reproductive ability and offspring development
Long-term toxicity of reduced graphene oxide nanosheets: Effects on female mouse reproductive ability and offspring development
复制标题
还原氧化石墨烯纳米片的长期毒性：对雌性小鼠生殖能力和后代发育的影响
DOI：
10.1016/j.biomaterials.2015.03.015
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发表时间：
2015
期刊：
影响因子：
14
通讯作者：
Chu Maoquan
中科院分区：
文献类型：
--
作者：
Xu Shun;Zhang Zheyu;Chu Maoquan
还原氧化石墨烯（rGO）纳米片已成为癌症治疗的新材料。由于这些纳米材料在临床癌症治疗中具有巨大的潜力，其毒性引起了人们的广泛关注。本研究报告了氧化石墨烯暴露对雌性小鼠生殖能力和后代发育的影响。在不同的剂量和时间点（受精前或受精后）注射小或大的氧化石墨烯纳米片。静脉注射小剂量或大剂量氧化石墨烯后，成年雌性小鼠的性激素水平与对照组相比没有显著变化，即使高剂量（25 mg/kg）。在妊娠前和妊娠早期（~ 6天）用氧化石墨烯纳米片处理后的小鼠可以产生健康的后代。尽管成功分娩的后代，畸形胎儿被发现在注射rgo的坝窝。在妊娠后期（~ 20天）注射低剂量（6.25 mg/kg）或中剂量（12.5 mg/kg）的小鼠均发生流产；大多数怀孕的老鼠在怀孕的这个阶段注射高剂量的氧化石墨烯后死亡。有趣的是，所有注射了氧化石墨烯的幸存小鼠母亲都产下了另一窝健康的幼崽。本研究的结果对于深入了解氧化石墨烯纳米片对雌性生殖能力及其后代发育的毒性具有重要意义。
Reduced graphene oxide (rGO) nanosheets have emerged as novel materials for cancer therapeutics. Their toxicity has attracted much attention since these nanomaterials may have great potential for clinical cancer treatment. Here we report the influence of rGO exposure on female mouse reproductive ability and offspring development. Mouse dams were injected with small or large rGO nanosheets at different doses and time points, pre- or post-fertilization. The sex hormone levels of adult female mice did not significantly change compared with the control group after intravenous injection with either small or large rGO, even at a high dose (25 mg/kg). Mouse dams could produce healthy offspring after treatment with rGO nanosheets before pregnancy and at an early gestational stage (∼6 days). Despite the successful delivery of offspring, malformed fetuses were found among rGO-injected dam litters. All mice had abortions when injected with low (6.25 mg/kg) or intermediate (12.5 mg/kg) doses at a late gestational stage (∼20 days); the majority of pregnant mice died when injected with the high dose of rGO at this stage of pregnancy. Interestingly, all surviving rGO-injected mouse mothers gave birth to another litter of healthy pups. The results presented in this work are important for a deeper understanding of the toxicity of rGO nanosheets on female reproductivity and their offspring development.