Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their blaNDM Genetic Contexts
Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their blaNDM Genetic Contexts
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DOI：
10.1128/aac.01243-15
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发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Sidjabat, Hanna E.
中科院分区：
文献类型：
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作者：
Wailan, Alexander M.;Paterson, David L.;Sidjabat, Hanna E.
bla（NDM）已在不同肠杆菌科物种和许多质粒复制子类型（Inc）中被报道。质粒复制子分型，结合细菌宿主的基因组特征（例如，序列分型），用于推断抗菌素耐药性决定因素在遗传无关的细菌宿主之间的传播。bla（NDM）的遗传背景是异质的。在这项研究中，我们对2012年至2014年在澳大利亚分离的12种产生ndm的肠杆菌科进行了基因组特征分析：大肠杆菌（n = 6）、肺炎克雷伯菌（n = 3）、阴沟肠杆菌（n = 2）和雷特氏普罗维登斯菌（n = 1）。我们在Tn125中描述了它们的blaNDM遗传背景，为bla（NDM）进入肠杆菌科提供了见解。incfii型（n = 7）和IncX3型（n = 4）质粒是最常见的质粒类型。IncHI1B （n = 1）质粒也被鉴定出来。鉴定出5种不同的bla（NDM）遗传背景，表明4种特定的质粒具有特定的bla（NDM）遗传背景（NGCs），其中3种为IncFII质粒（FII-A至-C）。值得注意的是，P. rettgeri的bla（NDM）遗传环境不是共轭的。我们的产ndm肠杆菌科之间的流行病学联系是通过获得这五种特定的质粒类型建立的。不同分子和遗传表征方法的结合使我们能够深入了解传播bla（NDM）的质粒的传播。
bla(NDM) has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. The genetic context of bla(NDM) is heterogeneous. In this study, we genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014: Escherichia coli (n = 6), Klebsiella pneumoniae (n = 3), Enterobacter cloacae (n = 2) and Providencia rettgeri (n = 1). We describe their blaNDM genetic contexts within Tn125, providing insights into the acquisition of bla(NDM) into Enterobacteriaceae. IncFII-type (n = 7) and IncX3 (n = 4) plasmids were the most common plasmid types found. The IncHI1B (n = 1) plasmid was also identified. Five different bla(NDM) genetic contexts were identified, indicating four particular plasmids with specific bla(NDM) genetic contexts (NGCs), three of which were IncFII plasmids (FII-A to -C). Of note, the bla(NDM) genetic context of P. rettgeri was not conjugative. Epidemiological links between our NDM-producing Enterobacteriaceae were established by their acquisition of these five particular plasmid types. The combination of different molecular and genetic characterization methods allowed us to provide insight into the spread of plasmids transmitting bla(NDM).