Sonic hedgehog N-terminal level correlates with adiponectin level and insulin resistance in adolescents
Sonic hedgehog N-terminal level correlates with adiponectin level and insulin resistance in adolescents
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DOI：
10.1515/jpem-2022-0385
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发表时间：
2023-01
影响因子：
1.4
通讯作者：
J. Takaya;Y. Tanabe;Kazunari Kaneko
中科院分区：
文献类型：
--
作者：
J. Takaya;Y. Tanabe;Kazunari Kaneko
摘要目的细胞外囊泡是由几乎所有类型的细胞释放的小囊泡。它们将不同类型的物质（包括蛋白质和核酸）传递到附近或远处的细胞，并在细胞间通讯的介导中发挥作用。本研究旨在探讨肥胖或2型糖尿病（DM 2）青少年EV与胰岛素抵抗的关系。方法研究对象为8例2型糖尿病（DM 2组，男4例，女4例，年龄18.1 ± 2.3岁），18例单纯性肥胖（肥胖组，男12例，女6例，年龄12.2 ± 3.4岁），20例对照（对照组，男10例，女10例，年龄13.0 ± 1.4岁）。作为EV的标志物，使用酶联免疫吸附试验测定血清CD 9/CD 63和音刺猬N-末端（Shh-N）水平。结果正常对照组CD 9/CD 63水平与DM 2组相似，而肥胖组CD 9/CD 63水平明显高于DM 2组。在整个研究组中，观察到血清Shh-N水平与胰岛素抵抗稳态模型评估（HOMA-IR）评分之间的相关性（r=0.371，p=0.0143），稳态模型评估-β细胞功能评分（r=0.382，p=0.0115）、血清胰岛素水平（r=0.350，p=0.0171）、血清脂联素水平（r=0.367，p=0.0122）。多元逐步回归分析显示，血清Shh-N水平是影响HOMA-IR评分和血清胰岛素水平的最显著危险因素。结论Shh通过与脂联素的相关性与青少年胰岛素抵抗相关。
Abstract Objectives Extracellular vesicles (EVs) are small vesicles released by nearly all types of cells. They deliver different types of substances, including proteins and nucleic acids, to nearby or distant cells and play a role in the mediation of cell-to-cell communication. The aim of this study was to explore the association between EVs and insulin resistance in adolescents with obesity or type 2 diabetes mellitus (DM2). Methods The subjects were eight adolescents with DM2 (DM2 group; four males and four females; age: 18.1 ± 2.3 years), 18 adolescents with simple obesity (obesity group; 12 males and six females; age: 12.2 ± 3.4 years), and 20 controls (control group; 10 males and 10 females; age: 13.0 ± 1.4 years). As markers of EVs, serum CD9/CD63 and sonic hedgehog N-terminal (Shh-N) levels were measured using enzyme-linked immunosorbent assay. Results The CD9/CD63 level in the control group was similar to that in the DM2 group, whereas the obesity group had a significantly higher CD9/CD63 level. In the entire study group, correlations were observed between serum Shh-N level and Homeostasis Model Assessment of insulin resistance (HOMA-IR) score (r=0.371, p=0.0143), Homeostasis Model Assessment-β cell function score (r=0.382, p=0.0115), serum insulin level (r=0.350, p=0.0171), and serum adiponectin level (r=0.367, p=0.0122). Multiple regression analysis revealed that serum Shh-N level was the most significant risk factor for HOMA-IR score and serum insulin level. Conclusions Shh is correlated with insulin resistance via its association with adiponectin in adolescents.