EVALUATION OF NESTED POLYMERASE CHAIN METHODS FOR THE DETECTION OF HUMAN CORONAVIRUSES 229E AND OC43
EVALUATION OF NESTED POLYMERASE CHAIN METHODS FOR THE DETECTION OF HUMAN CORONAVIRUSES 229E AND OC43
复制标题
DOI：
10.1006/mcpr.1994.1052
复制
发表时间：
1994-10-01
影响因子：
3.3
通讯作者：
SIMPSON, H
中科院分区：
文献类型：
--
作者：
MYINT, S;JOHNSTON, S;SIMPSON, H
目前，人类呼吸道冠状病毒感染的诊断要么是stow要么是不敏感的。本文介绍了巢式聚合酶链反应检测人类冠状病毒OC 43和229 E。该方法的特异性和灵敏度已被确定，并已应用于鼻吸出物中病毒的检测。这些测定比细胞培养更快速和灵敏，并可能取代后者作为诊断方法的选择。
Currently, the diagnosis of human respiratory coronavirus infection is either stow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice.