STRUCTURAL-ANALYSIS OF CYCLAMEN SEED XYLOGLUCAN OLIGOSACCHARIDES USING CELLULASE DIGESTION AND SPECTROSCOPIC METHODS
STRUCTURAL-ANALYSIS OF CYCLAMEN SEED XYLOGLUCAN OLIGOSACCHARIDES USING CELLULASE DIGESTION AND SPECTROSCOPIC METHODS
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DOI：
10.1016/0008-6215(95)00156-n
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发表时间：
1995-10-16
影响因子：
3.1
通讯作者：
GAGE, DA
中科院分区：
文献类型：
--
作者：
BRACCINI, I;DUPENHOAT, CH;GAGE, DA
从仙客来种子中分离得到木葡聚糖聚合物，并用液体和CP-MAS C-13核磁共振波谱对其结构进行了表征。绿色木霉的纤维素酶处理得到了XG低聚物，并用质谱仪和核磁共振谱对其进行了研究。完整聚合物中的重复单元和最丰富的水解产物对应于Gal(1)Xyl(3)Glc(4)(XXLG)片段。然而，检测到大量的XYL(3)GLC(4)(XXXG)和Gal(2)XYL(3)GLC(4)(XLLG)表明仙客来木聚糖中的半乳糖分布是不规则的。对间四氟乙氧基苯胺的糖胺形成的一种新的衍生物进行FAB-MS分析，得到了XXLG齐聚物的明确序列信息。
Xyloglucan polymers have been isolated from cyclamen seeds and characterized by both liquid and CP-MAS C-13 NMR spectroscopy. Treatment of the polysaccharides with cellulase from Trichoderma viride afforded XG oligomers which have been studied with both mass spectrometry and NMR spectroscopy. The repeating unit in the intact polymers and the most abundant hydrolysis product correspond to the Gal(1) Xyl(3) Glc(4) (XXLG) fragment. However, detection of notable amounts of Xyl(3) Glc(4) (XXXG) and Gal(2) Xyl(3) Glc(4) (XLLG) indicates that the galactose distribution in xyloglucan from cyclamen is irregular. FAB-MS analysis of a new derivative, prepared by forming the glycosamine of m-tetrafluoroethoxy aniline, has led to unambiguous sequence information for the XXLG oligomer.