Use of micromolded carbon dual electrodes with a palladium decoupler for amperometric detection in microchip electrophoresis
Use of micromolded carbon dual electrodes with a palladium decoupler for amperometric detection in microchip electrophoresis
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DOI：
10.1002/elps.200600401
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发表时间：
2006-12-01
期刊：
影响因子：
2.9
通讯作者：
Martin, R. Scott
中科院分区：
文献类型：
--
作者：
Mecker, Laura C.;Martin, R. Scott
本文描述了微模双碳墨电极的制作和评价，以及它们与用于微芯片电泳的钯电极的集成。与先前涉及具有微芯片电泳的碳基双电极的工作相反，这种方法导致电极以其中偶联器/电极对准是固定的和可再现的方式适合于大规模生产。在这项工作中，电极的尺寸和间距进行了优化，导致双碳电极的高度为1 μ m，间隔100 μ m。荧光显微镜被用来调查泄漏周围的电极/通道接口，以及调查什么效果的双电极上的频带展宽现象。通过在抗坏血酸存在下对神经递质的分离和选择性双电极检测来证明微电极的性能。还发现向缓冲系统中添加SDS改善了LOD和收集效率。这种方法是第一种涉及碳基双电极与片上钯离子的方法，将有助于分离和检测通过体内采样收集或从片上细胞释放的神经递质。
The fabrication and evaluation of micromolded dual carbon ink electrodes and their integration with a fabricated palladium decoupler for use in microchip electrophoresis is described. As opposed to previous work involving carbon-based dual electrodes with microchip electrophoresis, this approach results in electrodes that are amenable to mass production in a manner where the clecoupler/electrode alignment is fixed and reproducible. In this work, electrode sizes and spacings were optimized to result in dual carbon electrodes that are 1 mu m in height and separated by 100 mu m. Fluorescence microscopy was used to investigate leakage around the electrode/channel interface as well as to investigate what effect the dual electrodes have on band broadening phenomena. The performance of the microelectrodes was demonstrated by the separation and selective dual electrode detection of neurotransmitters in the presence of ascorbic acid. It was also found that addition of SDS to the buffer system improved both the LODs and collection efficiencies. This approach, which is the first involving carbon-based dual electrodes with an on-chip palladium decoupler, will be useful for separating and detecting neurotransmitters that are either collected by in vivo sampling or released from cells on-chip.