Patterns of large-scale genomic variation in virulent and avirulent Burkholderia species
Patterns of large-scale genomic variation in virulent and avirulent Burkholderia species
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DOI：
10.1101/gr.1608904
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发表时间：
2004-11-01
期刊：
影响因子：
7
通讯作者：
Tan, P
中科院分区：
文献类型：
--
作者：
Ong, C;Ooi, CH;Tan, P
人类疾病类鼻疽和鼻疽分别由类鼻疽伯克霍尔德氏菌和鼻疽伯克霍尔德氏菌引起，这两种细菌都被视为潜在的生物战剂。我们使用拟鼻疽伯克霍尔德氏菌 DNA 微阵列比较了拟鼻疽伯克氏菌、鼻疽伯克氏菌和泰国鼻疽伯克氏菌（一种密切相关但无毒力的物种）的几种临床和环境分离株的基因组。三个物种之间删除的开放阅读框（ORF）与不同的细胞功能相关，包括氮和铁代谢、群体感应和多糖产生。 B. mallei 中删除的 ORF 表现出显着的基因组聚类，而 B. thailandensis 中的删除则更加均匀地分散，这表明 B. mallei 和 B. thailandensis 可能通过不同的机制与 B. pseudomallei 产生分歧。独立的类鼻疽伯克氏菌分离株的基因组高度保守，分离株之间的大规模变异小于 3%，并且至少可以定义三种不同的分子亚型。对亚型特异性基因组区域的分析表明，DNA 丢失在自然环境中类鼻疽杆菌的进化辐射中发挥了重要作用。我们的结果提出了一些关于伯克霍尔德氏菌家族成员表现出的多种生物学特性的可能机制的假设。
The human diseases melioidosis and glanders are caused by the bacteria Burkholderia pseudomallei and B. mallei respectively, and both species are regarded as potential biowarfare agents. We used B. pseudomallei DNA microarrays to compare the genomes of several clinical and environmental isolates of B. pseudomallei, B. mallel, and B. thailandensis, a closely related but avirulent species. Open reading frames (ORFs) deleted between the three species were associated with diverse cellular functions, including nitrogen and iron metabolism, quorum sensing, and polysaccharide production. Deleted ORFs in B. mallei exhibited significant genomic clustering, whereas deletions in B. thailandensis were more uniformly dispersed, suggesting that B. mallei and B. thailandensis may have diverged from B. pseudomallei and each other via distinct mechanisms. The genomes of independent B. pseudomallei isolates were highly conserved with a large-scale variance of less than 3% between isolates, and at least three distinct molecular subtypes could be defined. An analysis of subtype-specific genomic regions suggests that DNA loss has played an important role in the evolutionary radiation of B. pseudomallei in the natural environment. Our results raise several hypotheses concerning the possible mechanisms underlying the diverse biological properties exhibited by members of the Burkholderia family.