Reproductive Fitness and Dietary Choice Behavior of the Genetic Model Organism Caenorhabditis elegans under Semi-Natural Conditions
Reproductive Fitness and Dietary Choice Behavior of the Genetic Model Organism Caenorhabditis elegans under Semi-Natural Conditions
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DOI：
10.1007/s10059-010-0125-9
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发表时间：
2010-10-01
影响因子：
3.8
通讯作者：
Paul, Ruediger J.
中科院分区：
文献类型：
--
作者：
Freyth, Katharina;Janowitz, Tim;Paul, Ruediger J.
模式生物秀丽隐杆线虫的实验室繁殖条件与其自然土壤生境条件不相适应。为了评估环境条件差异的后果，研究了空气成分、培养基和细菌食物对秀丽隐杆线虫繁殖适宜性和/或饮食选择行为的影响。秀丽隐杆线虫的生殖适合度在缺氧条件下达到最大，而不受高浓度二氧化碳的影响。在接近自然土壤结构的培养基中，繁殖适宜度远低于标准实验室培养基。在半自然培养基中，秀丽隐杆线虫以标准实验室食用细菌大肠杆菌（γ -变形菌门）为繁殖适宜度较低，而以拟杆菌门C. arvensicola和β -变形菌门B. tropica为繁殖适宜度显著较高。在半自然培养基中进行的饮食选择实验显示，秀丽隐杆线虫对大肠杆菌的偏好较低，但对葡萄球菌和热带芽孢杆菌（以及其他细菌）的偏好明显较高。细菌荧光原位杂交（FISH）在准自然条件下进行的饮食选择实验表明，秀丽隐杆线虫对胞噬-挠性杆菌-拟杆菌、厚壁菌门和β -变形菌门有较高的偏好，但对γ -变形菌门的偏好较低。结果表明，秀丽隐杆线虫在标准实验室条件下的数据必须仔细解释其生物学意义。
Laboratory breeding conditions of the model organism C. elegans do not correspond with the conditions in its natural soil habitat. To assess the consequences of the differences in environmental conditions, the effects of air composition, medium and bacterial food on reproductive fitness and/or dietary-choice behavior of C. elegans were investigated. The reproductive fitness of C. elegans was maximal under oxygen deficiency and not influenced by a high fractional share of carbon dioxide. In media approximating natural soil structure, reproductive fitness was much lower than in standard laboratory media. In semi-natural media, the reproductive fitness of C. elegans was low with the standard laboratory food bacterium E. coli (gamma-Proteobacteria), but significantly higher with C. arvensicola (Bacteroidetes) and B. tropica (beta-Proteobacteria) as food. Dietary-choice experiments in semi-natural media revealed a low preference of C. elegans for E. coli but significantly higher preferences for C. arvensicola and B. tropica (among other bacteria). Dietary-choice experiments under quasi-natural conditions, which were feasible by fluorescence in situ hybridization (FISH) of bacteria, showed a high preference of C. elegans for Cytophaga-Flexibacter-Bacteroides, Firmicutes, and beta-Proteobacteria, but a low preference for gamma-Proteobacteria. The results show that data on C. elegans under standard laboratory conditions have to be carefully interpreted with respect to their biological significance.