Effects of prenatal ethanol exposure on brain region NMDA-mediated increase in intracellular calcium and the NMDAR1 subunit in forebrain.
Effects of prenatal ethanol exposure on brain region NMDA-mediated increase in intracellular calcium and the NMDAR1 subunit in forebrain.
复制标题
DOI：
10.1111/j.1530-0277.1997.tb03730.x
复制
发表时间：
1997-02
期刊：
影响因子：
--
通讯作者：
K. Spuhler-Phillips;Young Hee Lee;P. D. Hughes;L. A. Randoll;Steven W. Leslie
中科院分区：
文献类型：
--
作者：
K. Spuhler-Phillips;Young Hee Lee;P. D. Hughes;L. A. Randoll;Steven W. Leslie
研究了产前乙醇暴露对N-甲基-D-天冬氨酸（NMDA）介导的新生儿海马、前脑和小脑分离神经元钙内流的影响。从产前暴露、配对喂养对照组和随意对照组的小于1日龄幼仔中分离解离细胞，并加载fura-2。与两个对照组相比，产前乙醇暴露显著降低了所有三个脑区中NMDA刺激的细胞内钙的增加。这些发现是非常相似的，以前观察到的新生儿分离的全脑神经元使用相同的乙醇暴露协议。还进行了研究，使用前脑，以确定是否产前乙醇暴露改变NMDAR 1亚基蛋白表达在这个主要的大脑区域，然而，结果表明乙醇暴露组和对照组之间没有显着差异。
The effects of prenatal ethanol exposure on N-methyl-D-aspartate (NMDA)-mediated calcium entry into neonatal dissociated neurons from hippocampus, forebrain, and cerebellum were investigated. Dissociated cells were isolated from less than 1-day-old pups of prenatally exposed, pair-fed control and ad libitum control groups and loaded with fura-2. Prenatal ethanol exposure significantly reduced the NMDA-stimulated increase in intracellular calcium in all three brain regions compared to the two control groups. These findings are very similar to those previously observed in neonatal dissociated whole brain neurons using the same ethanol exposure protocol. Studies were also conducted using forebrain to determine if prenatal ethanol exposure alters NMDAR1 subunit protein expression in this major brain area; however, the results indicated no significant differences between ethanol-exposed and control groups.