Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds
Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds
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DOI：
10.1007/s11130-009-0133-0
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发表时间：
2009-12-01
影响因子：
4
通讯作者：
Menichini, Francesco
中科院分区：
文献类型：
--
作者：
Loizzo, Monica Rosa;Said, Ataa;Menichini, Francesco
本研究的目的是确定黑枣提取物的化学成分及其抗氧化和抗增殖特性。从 D. Lotus 中分离出八种化合物，并鉴定为没食子酸、没食子酸甲酯、鞣花酸、山奈酚、槲皮素、杨梅素、杨梅素 3-O-β-葡萄糖醛酸和杨梅素-3-O-α-鼠李糖苷。 D.莲花提取物在不同的体外系统（DPPH、ABTS、FRAP 和 Fe2+ 螯合活性测定）中测试显示出显着的抗氧化活性。通过 SRB 测定，在体外研究了莲花提取物和分离化合物针对 COR-L23、CaCo-2、C32、ACHN、A375、A549、Huh-7D12、MCF-7 和 LNCaP 等 9 种人类癌细胞系的潜在抗增殖特性。 D. Lotus提取物对COR-L23表现出最高的抑制活性，IC50值为12.2 μg/ml。在已鉴定的可水解单宁中，鞣花酸对 C32 和 A375 细胞具有很强的抗增殖活性，IC50 值分别为 0.8 和 4.1 μg/ml。还观察到了有趣的结果，没食子酸对 CaCo-2 表现出最高的细胞毒活性（IC50 2.6 μg/ml）。总体而言，本研究结果表明，莲花具有良好的抗氧化活性并具有抗增殖作用。这两种活性都与已确定的酚类化合物有关。
The object of the study was to determine the chemical composition of Diospyros lotus L. extract and their antioxidant and antiproliferative properties. Eight compounds were isolated from D. lotus and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin, myricetin, myricetin 3-O-beta-glucuronide, and myricetin-3-O-alpha-rhamnoside. D. lotus extract tested in different in vitro systems (DPPH, ABTS, FRAP, and Fe2+ chelating activity assay) showed significant antioxidant activity. The potential antiproliferative properties of D. lotus extract and isolated compounds against nine human cancer cell lines such as COR-L23, CaCo-2, C32, ACHN, A375, A549, Huh-7D12, MCF-7, and LNCaP were investigated in vitro by SRB assay. D. lotus extract demonstrated the highest inhibitory activity against COR-L23 with an IC50 value of 12.2 mu g/ml. Among identified hydrolysable tannins, ellagic acid evidenced strong antiproliferative activity against both C32 and A375 cells with IC50 values of 0.8 and 4.1 mu g/ml, respectively. Interesting results were observed, also, with gallic acid that showed the highest cytotoxic activity against CaCo-2 (IC50 2.6 mu g/ml). Overall, the results of this study suggest that D. lotus displays a good antioxidant activity and has antiproliferative effects. Both activities are related to identified phenolic compounds.