SHIFTS IN THE CONCENTRATIONS OF MAGNESIUM AND CALCIUM IN EARLY PORCINE AND RAT WOUND FLUIDS ACTIVATE THE CELL MIGRATORY RESPONSE
SHIFTS IN THE CONCENTRATIONS OF MAGNESIUM AND CALCIUM IN EARLY PORCINE AND RAT WOUND FLUIDS ACTIVATE THE CELL MIGRATORY RESPONSE
复制标题
DOI：
10.1172/jci117644
复制
发表时间：
1995-01-01
影响因子：
15.9
通讯作者：
PIERSCHBACHER, MD
中科院分区：
文献类型：
--
作者：
GRZESIAK, JJ;PIERSCHBACHER, MD
越来越多的证据表明，细胞外Mg 2+和Ca 2+的水平可以对许多细胞类型的粘附和迁移活动产生明显的影响。然而，这些观察结果的生理相关性在很大程度上仍未被探索。在本研究中，在皮肤伤口修复的早期阶段收集的伤口液进行了检查，可能的Mg 2+和Ca 2+的波动。在该过程的早期，当细胞开始迁移到伤口部位时，Mg 2+升高，Ca 2+降低（Mg 2+：Ca 2 + = 1）。随着伤口愈合的进展，伤口流体中Mg 2+和Ca 2+的浓度开始恢复到正常血浆水平（Mg 2+：Ca 2 + = 0.4）。当巨噬细胞、角质形成细胞、成纤维细胞和内皮细胞暴露于透析伤口液时，未透析伤口液刺激的迁移丧失。此外，回透析伤口液的24小时，伤后浓度的Mg 2+和Ca 2+恢复所有迁移刺激。这种观察到的迁移大约是正常血浆Mg 2+和Ca 2+浓度的两倍。伤口液中Mg 2+和Ca 2+水平的变化发生在与炎症细胞、角质形成细胞、成纤维细胞和新血管系统已显示在体内伤口愈合期间迁移的相同时期。总之，这些数据表明，这些变化对整合素和E-钙粘蛋白的影响可能在激活和维持参与伤口愈合过程的细胞的迁移表型中发挥直接作用。
Accruing evidence indicates that the levels of extracellular Mg2+ and Ca2+ can have a distinct impact on the adhesive and migratory activities of many cell types. The physiological relevance of these observations, however, has remained largely unexplored. In the present study, wound fluids collected throughout the early stages of cutaneous wound repair were examined for possible Mg2+ and Ca2+ fluctuations. Early in the process, when cell migration into the wound site is initiated, Mg2+ is elevated and Ca2+ is reduced (Mg2+:Ca2+ = 1). As wound healing progresses, wound fluid concentrations of Mg2+ and Ca2+ begin to return to normal plasma levels (Mg2+:Ca2+ = 0.4). When macrophages, keratinocytes, fibroblasts, and endothelial cells were exposed to dialyzed wound fluid, the migration stimulated by undialyzed wound fluid was lost. Addition back to dialyzed wound fluid of 24 h, postinjury concentrations of Mg2+ and Ca2+ restored all migratory stimulus. This observed migration is approximately twofold greater than when normal plasma Mg2+ and Ca2+ concentrations are present. Changes in the levels of Mg2+ and Ca2+ in wound fluid occur during the same period that inflammatory cells, keratinocytes, fibroblasts, and neovasculature have been shown to migrate during wound healing in vivo. Together, these data suggest that the impact of these changes on integrins and E-cadherin may play a direct role in the activation and maintenance of the migratory phenotypes of the cells involved in the wound healing process.