Open-Ended Inquiry into Zebrafish Nerve Development Using Image Analysis.
Open-Ended Inquiry into Zebrafish Nerve Development Using Image Analysis.
复制标题
使用图像分析对斑马鱼神经发育进行开放式调查。
DOI：
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复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sarah C. Petersen
中科院分区：
文献类型：
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作者：
Sarah C. Petersen
开放式实验室项目提高学生在科学方面的成功和保留。然而，开发基于生物的研究项目对于实验室访问受限的学生来说是一个挑战，例如远程参加课程的学生。在这里，我描述了使用图像分析的斑马鱼神经发育的真实的研究项目，在介绍生物学实验室课程。斑马鱼是一种脊椎动物模型，可以产生大量的外部和快速发育的胚胎。由于斑马鱼幼虫是透明的，标记神经系统结构的荧光报告基因可以随着时间的推移进行成像并由本科科学家进行分析。在该项目的试点中，远程一年级的大学生根据比较斑马鱼突变体和野生型兄弟姐妹的图像集独立开发生物学问题。学生们创造并掌握了分析图像中发育中的神经元和神经胶质的位置、组织和其他形态特征的技术，以直接测试他们的生物学问题。在课程结束时，学生们以期刊文章的形式和口头报告的形式交流了他们的项目结果。学生们能够在远程学习的同时磨练生物观察和数据收集的技能，他们报告说，将基于讲座的知识应用于自己的独立问题时感到兴奋。该模块可以由其他教师为校内和校外的学生进行调整，以教授神经发育，数据收集，数据分析和科学交流的原则。
Open-ended laboratory projects increase student success and retention in the sciences. However, developing organismal-based research projects is a challenge for students with restricted laboratory access, such as those attending courses remotely. Here I describe the use of image analysis of zebrafish neural development for authentic research projects in an introductory biology laboratory course. Zebrafish are a vertebrate model that produce large numbers of externally and rapidly developing embryos. Because zebrafish larvae are transparent, fluorescent reporters marking nervous system structures can be imaged over time and analyzed by undergraduate scientists. In the pilot of this project, remote first-year college students independently developed biological questions based on an image collection comparing zebrafish mutants and wild-type siblings. Students created and mastered techniques to analyze position, organization, and other morphological features of developing neurons and glia in the images to directly test their biological questions. At the end of the course, students communicated their project results in journal article format and oral presentations. Students were able to hone skills in organismal observation and data collection while studying remotely, and they reported excitement at applying lecture-based knowledge to their own independent questions. This module can be adapted by other instructors for both students on- and off-campus to teach principles of neural development, data collection, data analysis, and scientific communication.
影响因子：
3.7
作者：
Indorf, Jane L.;Weremijewicz, Joanna;Gaines, Michael S.
通讯作者：
Gaines, Michael S.
影响因子：
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作者：
D'Rozario M;Monk KR;Petersen SC
通讯作者：
Petersen SC
在没有正常先锋轴突的情况下斑马鱼运动神经元的寻路。
DOI：
10.1242/dev.114.4.825
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Pike,SH;Melancon,EF;Eisen,JS
通讯作者：
Eisen,JS