INHIBITORY EFFECT OF DIBUTYRYL CAMP ON MOUSE OOCYTE MATURATION IN-VITRO
INHIBITORY EFFECT OF DIBUTYRYL CAMP ON MOUSE OOCYTE MATURATION IN-VITRO
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DOI：
10.1002/jez.1401870307
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发表时间：
1974-01-01
影响因子：
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通讯作者：
BIGGERS, JD
中科院分区：
文献类型：
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作者：
CHO, WK;STERN, S;BIGGERS, JD
研究了二丁酰环AMP对小鼠卵母细胞体外成熟的影响。研究发现，100 μg/ml 或更高的浓度可防止 95% 以上的处理卵母细胞发生生发囊泡破裂。发现成熟的抑制是可逆的现象。当卵母细胞在生发囊泡分解完成后暴露于药物时，没有注意到药物的作用。因此，二丁酰环AMP似乎仅影响小鼠卵母细胞减数分裂成熟的初始阶段。茶碱也被发现抑制成熟，这表明卵母细胞中环AMP水平的增加可能是成熟抑制的原因。
The effects of dibutyryl cyclic AMP on the maturation of the mouse oocyte in vitro were studied. It was found that concentrations of 100 μg/ml or greater prevented germinal vesicle breakdown in more than 95% of the treated oocytes. The inhibition of maturation was found to be a reversible phenomenon. When oocytes were exposed to the drug after the completion of germinal vesicle breakdown, no effect of the drug was noted. Thus, dibutyryl cyclic AMP appears to effect only the initial stages of meiotic maturation in the mouse oocyte.Theophylline was also found to inhibit maturation, suggesting that increased levels of cyclic AMP in the oocyte may be the causative agent of the inhibition of maturation.