LOCALIZATION OF VIMENTIN, THE NONSPECIFIC INTERMEDIATE FILAMENT PROTEIN, IN EMBRYONAL GLIA AND IN EARLY DIFFERENTIATING NEURONS - INVIVO AND INVITRO IMMUNOFLUORESCENCE STUDY OF THE RAT EMBRYO WITH VIMENTIN AND NEUROFILAMENT ANTISERA
LOCALIZATION OF VIMENTIN, THE NONSPECIFIC INTERMEDIATE FILAMENT PROTEIN, IN EMBRYONAL GLIA AND IN EARLY DIFFERENTIATING NEURONS - INVIVO AND INVITRO IMMUNOFLUORESCENCE STUDY OF THE RAT EMBRYO WITH VIMENTIN AND NEUROFILAMENT ANTISERA
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DOI：
10.1016/0012-1606(82)90035-5
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
DAHL, D
中科院分区：
文献类型：
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作者：
BIGNAMI, A;RAJU, T;DAHL, D
抗波形蛋白，非特异性的中间大小的丝（IF）的蛋白质亚基提出的抗血清，与神经丝（NF）抗血清一起使用，以研究在大鼠胚胎神经元和胶质细胞的早期发育。在第12天首次观察到跨越神经管包括脑泡的整个厚度的波形蛋白阳性纤维，伴随着NF蛋白在更有限的区域（脊髓和脑干的前外侧区域;从NF阳性区域出现的运动根）中的出现。从第15天起，波形蛋白和NF抗血清选择性地装饰胶质细胞和神经元，分别在体内和体外。在第15天之前，在冷冻切片中，NF阳性结构似乎也被抗波形蛋白染色。体外实验证实了波形蛋白在早期分化的神经元中的存在。观察到NF阳性细胞也与抗波形蛋白在从13和14天的胚胎获得的培养物中反应，但在发育后期不反应。这些培养物中的大多数神经元在体外培养2-3天后变为波形蛋白阴性。
Antisera raised against vimentin, the protein subunit of nonspecific intermediate-sized filaments (IF), were used in conjunction with neurofilament (NF) antisera to study the early development of neurons and glia in the rat embryo. Vimentin-positive fibers spanning the entire thickness of the neural tube including the cerebral vesicles were first observed on day 12, concomitant with the appearance of NF protein in more confined areas (anterolateral regions of spinal cord and brain stem; motor roots emerging from the NF-positive areas). From day 15 onward, vimentin and NF antisera selectively decorated glia and neurons, respectively, in vivo and in vitro. Before day 15 it appeared that NF-positive structures also stained with antivimentin in cryostat sections. In vitro experiments confirmed the presence of vimentin in early differentiating neurons. NF-positive cells were observed which also reacted with antivimentin in cultures obtained from 13- and 14-day embryos, but not later in development. Most neurons in these cultures became vimentin-negative after 2-3 days in vitro.