A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato
A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato
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DOI：
10.1016/j.cell.2019.11.037
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发表时间：
2020-01-09
期刊：
影响因子：
64.5
通讯作者：
Sattely, Elizabeth
中科院分区：
文献类型：
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作者：
Jeon, Ju Eun;Kim, Jung-Gun;Sattely, Elizabeth
为了应对生物胁迫，植物产生一系列高度修饰的脂肪酸，这些脂肪酸具有不寻常的化学功能。尽管它们的化学复杂性和在病原体防御中的作用，但对植物中修饰脂肪酸的生物合成知之甚少。Falcarindiol是存在于胡萝卜、番茄和芹菜中的原型炔属脂质，其抑制真菌和人类癌细胞系的生长。使用非靶向代谢组学和RNA测序的组合，我们发现了一个生物合成基因簇在番茄（番茄）所需的falcarindiol生产。通过在异源宿主中重建初始生物合成步骤并在番茄中产生转基因途径突变体，我们证明了簇在番茄叶中的镰孢二醇生物合成和对真菌和细菌病原体的抗性中的直接作用。这项工作揭示了一种机制，植物塑造他们的脂质池响应病原体，并提供了关键的洞察炔基脂质生产的复杂的生物化学。
In response to biotic stress, plants produce suites of highly modified fatty acids that bear unusual chemical functionalities. Despite their chemical complexity and proposed roles in pathogen defense, little is known about the biosynthesis of decorated fatty acids in plants. Falcarindiol is a prototypical acetylenic lipid present in carrot, tomato, and celery that inhibits growth of fungi and human cancer cell lines. Using a combination of untargeted metabolomics and RNA sequencing, we discovered a biosynthetic gene cluster in tomato (Solanum lycopersicum) required for falcarindiol production. By reconstituting initial biosynthetic steps in a heterologous host and generating transgenic pathway mutants in tomato, we demonstrate a direct role of the cluster in falcarindiol biosynthesis and resistance to fungal and bacterial pathogens in tomato leaves. This work reveals a mechanism by which plants sculpt their lipid pool in response to pathogens and provides critical insight into the complex biochemistry of alkynyl lipid production.