Phylogenetic relationships among Artemisia species based on nuclear ITS and chloroplast psbA-trnH DNA markers
Phylogenetic relationships among Artemisia species based on nuclear ITS and chloroplast psbA-trnH DNA markers
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DOI：
10.2478/s11756-014-0379-3
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发表时间：
2014-07-01
期刊：
影响因子：
1.5
通讯作者：
Terzioglu, Salih
中科院分区：
文献类型：
--
作者：
Haghighi, Ahmad Razban;Belduz, Ali Osman;Terzioglu, Salih
文章题目蒿属的分类和系统发育关系。（菊科）是有争议的，它已被认为是1至8个不同的属。本研究以蒿属（Artemisia）、龙蒿属（Dracunculus）和绢蒿属（Serphidium）三个组为材料，利用核核糖体（ITS）和叶绿体psbA-trnH DNA序列，对蒿属的系统发育关系进行了重新研究。利用最大简约法分别对ITS序列、psbA-trnH序列和组合序列构建了系统发育树。结果表明，这三个组彼此之间有明显的分离，异花配合的龙蒿属和蒿属之间的亲缘关系较近，而与同花配合的Serphidium之间的亲缘关系较远。这可能表明头状花序形态在蒿属的分类学上的重要性。我们的数据也对细胞遗传学相似性的使用提出了质疑，例如，Serphidium和Artemisia s.s. AMOVA分析表明，各区段间ITS（55.29%）和ITS+cppsbA-trnH组合（55.63%）的变异水平较高。这为这三个部分的分离提供了进一步的证据，并支持系统发育的结果。蒿属的ITS核苷酸差异较大（30.4737），而龙蒿属（2.3333）和Serphidium（1.23077）的差异很小，这可能表明蒿属由几个早期组组成。这支持了先前的建议，蒿是一个复杂的群体。
The taxonomic and phylogenetic relationships within the genus Artemisia s.l. (Asteraceae) are controversial, and it has been considered 1 to 8 different genera. This work re-investigated the phylogenetic relationships in Artemisia using nuclear ribosomal (ITS) and chloroplast psbA-trnH DNA sequences using three sections of Artemisia, Dracunculus, and Serphidium. Three phylogenetic trees were conducted separately on the basis of ITS, psbA-trnH and combined sequences using maximum parsimony. The results showed that the three sections were clearly separated from each other, and that the heterogamous Dracunculus and Artemisia are closely related to each other than either to homogamous Serphidium. This may suggest the taxonomic importance of capitulum morphology in Artemisia s.l. Our data also cast doubt on the use of cytogenetic similarity e.g., basic chromosome number in grouping Serphidium and Artemisia s.s. Furthermore, AMOVA analysis showed a higher level of ITS (55.29%) and combined ITS+cppsbA-trnH (55.63%) variations among sections. This provides further evidence for separation of these three sections and supports the phylogenetic results. The higher ITS nucleotide differences detected in Artemisia (30.4737) compared to very low value in Dracunculus (2.3333) and Serphidium (1.23077) may propose that the Artemisia comprises of several incipient sections. This supports the previous suggestion that Artemisia is a complex group.