Microbiota at Multiple Body Sites during Pregnancy in a Rural Tanzanian Population and Effects of Moringa-Supplemented Probiotic Yogurt
Microbiota at Multiple Body Sites during Pregnancy in a Rural Tanzanian Population and Effects of Moringa-Supplemented Probiotic Yogurt
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DOI：
10.1128/aem.00780-15
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发表时间：
2015-08-01
影响因子：
4.4
通讯作者：
Reid, Gregor
中科院分区：
文献类型：
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作者：
Bisanz, Jordan E.;Enos, Megan K.;Reid, Gregor
孕妇的营养状况对健康结果至关重要，是世界上很大一部分人口所关切的问题。微生物群在怀孕和营养中的作用是一个有前途的新研究领域，具有潜在的健康影响。在许多非洲国家，产妇和婴儿死亡和发病与营养不良有关。在这里，我们评估了含有鼠李糖乳杆菌GR-1的益生菌酸奶，补充辣木植物作为微量营养素来源，对坦桑尼亚56名孕妇的健康和口腔，肠道，阴道和牛奶微生物的影响。在一项开放标签研究设计中，26名受试者每天接受酸奶，30名受试者在最后两个孕期和出生后1个月内未接受治疗。使用16 S rRNA基因测序分析样品，并记录饮食回忆。最初被归类为营养不良或营养不良的女性消耗了类似的卡路里和大量营养素，这可能解释了为什么身体任何部位的微生物群都没有差异。酸奶的消费增加了新生儿粪便中双歧杆菌的相对丰度，减少了肠杆菌科，但对母亲身体任何部位的微生物群没有影响。口腔和胃肠道的微生物群在怀孕期间保持稳定，但阴道微生物群在出生前和出生后的多样性显着增加。总之，每日补充微量营养素的益生菌酸奶为坦桑尼亚农村的孕妇提供了一种安全、负担得起的食物，由此产生的婴儿肠道微生物状况的改善值得进一步研究。
The nutritional status of pregnant women is vital for healthy outcomes and is a concern for a large proportion of the world's population. The role of the microbiota in pregnancy and nutrition is a promising new area of study with potential health ramifications. In many African countries, maternal and infant death and morbidity are associated with malnutrition. Here, we assess the influence of probiotic yogurt containing Lactobacillus rhamnosus GR-1, supplemented with Moringa plant as a source of micronutrients, on the health and oral, gut, vaginal, and milk microbiotas of 56 pregnant women in Tanzania. In an open-label study design, 26 subjects received yogurt daily, and 30 were untreated during the last two trimesters and for 1 month after birth. Samples were analyzed using 16S rRNA gene sequencing, and dietary recalls were recorded. Women initially categorized as nourished or undernourished consumed similar calories and macronutrients, which may explain why there was no difference in the microbiota at any body site. Consumption of yogurt increased the relative abundance of Bifidobacterium and decreased Enterobacteriaceae in the newborn feces but had no effect on the mother's microbiota at any body site. The microbiota of the oral cavity and GI tract remained stable over pregnancy, but the vaginal microbiota showed a significant increase in diversity leading up to and after birth. In summary, daily micronutrient-supplemented probiotic yogurt provides a safe, affordable food for pregnant women in rural Tanzania, and the resultant improvement in the gut microbial profile of infants is worthy of further study.