Multivariate data analysis and metabolic profiling of artemisinin and related compounds in high yielding varieties of Artemisia annua field-grown in Madagascar.
Multivariate data analysis and metabolic profiling of artemisinin and related compounds in high yielding varieties of Artemisia annua field-grown in Madagascar.
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DOI：
10.1016/j.jpba.2015.10.003
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发表时间：
2016-01
影响因子：
3.4
通讯作者：
J. Suberu;P. Gromski;A. Nordon;A. Lapkin
中科院分区：
文献类型：
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作者：
J. Suberu;P. Gromski;A. Nordon;A. Lapkin
建立了一种改进的液相色谱-串联质谱（LC-MS/MS）快速分析A.开发并广泛表征了annuain粗提取物。用该方法分析了13个A.每年从马达加斯加的实地增长计划。几种多变量数据分析技术一致显示青蒿素与双氢青蒿酸的关联。这些数据支持双氢青蒿酸是青蒿素生物合成途径中的晚期前体的假设。
An improved liquid chromatography-tandem mass spectrometry (LC–MS/MS) protocol for rapid analysis of co-metabolites ofA. annuain raw extracts was developed and extensively characterized. The new method was used to analyse metabolic profiles of 13 varieties ofA. annuafrom an in-field growth programme in Madagascar. Several multivariate data analysis techniques consistently show the association of artemisinin with dihydroartemisinic acid. These data support the hypothesis of dihydroartemisinic acid being the late stage precursor to artemisinin in its biosynthetic pathway.