MONOCLONAL-ANTIBODY TO MURINE EMBRYOS DEFINES A STAGE-SPECIFIC EMBRYONIC ANTIGEN EXPRESSED ON MOUSE EMBRYOS AND HUMAN TERATOCARCINOMA CELLS
MONOCLONAL-ANTIBODY TO MURINE EMBRYOS DEFINES A STAGE-SPECIFIC EMBRYONIC ANTIGEN EXPRESSED ON MOUSE EMBRYOS AND HUMAN TERATOCARCINOMA CELLS
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DOI：
10.1016/0092-8674(82)90274-4
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
SOLTER, D
中科院分区：
文献类型：
--
作者：
SHEVINSKY, LH;KNOWLES, BB;SOLTER, D
小鼠阶段特异性胚胎抗原（SSEA3）是通过与4- 8细胞期小鼠胚胎免疫大鼠制备的单克隆抗体的反应性来定义的。这种抗原决定因素存在于卵母细胞中，在囊胚阶段首先局限于内细胞群，后来局限于原始内胚层。小鼠畸胎瘤干细胞不与这种抗体发生反应；人畸胎癌干细胞是ssea3阳性。这种抗原决定因素不表达在各种其他人类和小鼠细胞系，但发现在人红细胞表面。它是一种碳水化合物，存在于细胞表面的糖脂和糖肽中。结果表明，利用制备的胚胎单克隆抗体鉴定阶段特异性抗原决定因子是可行的。还强调需要对各种细胞类型进行彻底筛选，以建立发育重要的交叉反应性。
A murine stage-specific embryonic antigen (SSEA3) is defined by reactivity with a monoclonal antibody prepared by immunization of a rat with 4- to 8-cell-stage mouse embryos. This antigenic determinant, present on oocytes, becomes restricted first to the inner cell mass at the blastocyst stage, and later to the primitive endoderm. Murine teratocarcinoma stem cells do not react with this antibody; human teratocarcinoma stem cells are SSEA3-positive. This antigenic determinant is not expressed on a variety of other human and murine cell lines, but is found on the surface of human erythrocytes. It is a carbohydrate and is present on both cell surface glycolipids and glycopeptides. Results demonstrate the feasibility of identifying stage-specific antigenic determinants with monoclonal antibody prepared against embryos. The need for thorough screening on a variety of cell types to establish developmentally important cross-reactivities is also emphasized.