Expression of zinc transporter ZnT7 in mouse superior cervical ganglion
Expression of zinc transporter ZnT7 in mouse superior cervical ganglion
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锌转运蛋白ZnT7在小鼠颈上神经节中的表达
DOI：
10.1016/j.autneu.2008.04.002
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发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Wang, Zhan-You
中科院分区：
文献类型：
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作者：
Gao, Hui-Ling;Xu, He;Wang, Zhan-You
上级颈神经节（SCG）神经元含有大量的锌离子，但对SCG中锌的稳态知之甚少。已知锌转运蛋白7（ZnT7，Slc30a7）是Slc30 ZnT家族的成员，参与将锌离子从细胞质动员到高尔基体中。在本研究中，我们研究了ZnT7和不稳定的锌离子在小鼠SCG的表达和定位，免疫组织化学，蛋白质印迹和体内锌硒自显影（AMG）。我们的免疫组织化学分析表明，ZnT7免疫反应在SCG神经元主要存在于核周区的神经元，这表明附属高尔基体。Western blot结果证实ZnT7蛋白在小鼠SCG中有表达。AMG反应产物显示具有与ZnT7免疫反应性相似的分布。这些观察结果支持的概念，ZnT7可能参与锌的运输，存储，并纳入锌的锌结合蛋白在高尔基体的小鼠SCG神经元。(C)2008 Elsevier B.V.保留所有权利。
The superior cervical ganglion (SCG) neurons contain a considerable amount of zinc ions, but little is known about the zinc homeostasis in the SCG. It is known that zinc transporter 7 (ZnT7, Slc30a7), a member of the Slc30 ZnT family, is involved in mobilizing zinc ions from the cytoplasm into the Golgi apparatus. In the present study, we examined the expression and localization of ZnT7 and labile zinc ions in the mouse SCG using immunohistochemistry, Western blot and in vivo zinc selenium autometallography (AMG). Our immunohistochemical analysis revealed that the ZnT7 immunoreactivity in the SCG neurons was predominately present in the perinuclear region of the neurons, suggesting an affiliation to the Golgi apparatus. The Western blot results verified that ZnT7 protein was expressed in the mouse SCGs. The AMG reaction product was shown to have a similar distribution as ZnT7 immunoreactivity. These observations support the notion that ZnT7 may participate in zinc transport, storage, and incorporation of zinc into zinc-binding proteins in the Golgi apparatus of mouse SCG neurons. (C) 2008 Elsevier B.V. All rights reserved.