THE COMPLEMENT FIXATION TEST IN THE DIAGNOSIS OF VIRUS INFECTIONS OF THE CENTRAL NERVOUS SYSTEM.
THE COMPLEMENT FIXATION TEST IN THE DIAGNOSIS OF VIRUS INFECTIONS OF THE CENTRAL NERVOUS SYSTEM.
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DOI：
10.1084/jem.74.5.409
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发表时间：
1941-10-31
影响因子：
15.3
通讯作者：
Palacios, R
中科院分区：
文献类型：
--
作者：
Casals, J;Palacios, R
在各种中枢神经系统病毒感染中，通过使用感染脑组织的乳剂作为抗原，冷冻和解冻脑乳剂，然后在3500 RPM的角头离心机中离心1小时，可以获得特异性补体结合试验。在狂犬病、圣路易斯脑炎、日本B脑炎、淋巴细胞性脉络丛脑膜炎、东方马脑脊髓炎、西方马脑脊髓炎、louping病和小鼠自发性脑脊髓炎（Theiler病）的情况下，证明该方法是可靠的。无论涉及何种病毒，反应的特异性都要求根据动物物种的不同，血清的灭活温度不同：56°C。对于豚鼠，60°C。对于小鼠，和65°C。对于兔和狗血清，全部加热20分钟。对于人血清，灭活温度为60°C。也采用了20分钟;在该温度下，反应通常是特异性的。用狂犬病毒免疫家兔、豚鼠、小鼠和犬后，其血清中均检测到高滴度的补体结合抗体。从两个人的血清中提取的补体结合抗体存在高滴度luping病发作后8年，从5人的东部马脑脊髓炎发作后2年半，从两个人的西部马脑脊髓炎发作后2年半。在圣路易斯脑炎和淋巴细胞性脉络膜脑膜炎的病例中，感染后不久就发现了补体固定抗体，但在很长一段时间后才发现。
A specific complement fixation test can be obtained in various central nervous system virus infections by using as antigens emulsions of infected brain tissue, freezing and thawing the brain emulsion, and then centrifuging it in an angle head centrifuge at 3500 R.P.M. for 1 hour. The method has proved reliable in the case of rabies, St. Louis encephalitis, Japanese B encephalitis, lymphocytic choriomeningitis, Eastern equine encephalomyelitis, Western equine encephalomyelitis, louping ill, and spontaneous encephalomyelitis of mice (Theiler's disease). The specificity of the reaction, regardless of the virus involved, requires different temperatures of inactivation of the sera according to animal species: 56°C. for guinea pig, 60°C. for mouse, and 65°C. for rabbit and dog sera, all heated for 20 minutes. For human sera a temperature of inactivation of 60°C. also for 20 minutes has been adopted; at this temperature the reaction is in general specific. Complement-fixing antibodies in high titre were found in the sera of rabbits, guinea pigs, mice, and dogs immunized with rabies virus. Complement-fixing antibodies were present in high titre in sera drawn from two persons 8 years after an attack of louping ill, from five persons 2½ years after an attack of Eastern equine encephalomyelitis, and from two persons 2½ years after Western equine encephalomyelitis. In cases of St. Louis encephalitis and lymphocytic choriomeningitis, complement-fixing antibodies have been found shortly following infection but not after long periods.