A MONOCLONAL-ANTIBODY AGAINST NEUROFILAMENT PROTEIN SPECIFICALLY LABELS A SUBPOPULATION OF RAT SENSORY NEURONS
A MONOCLONAL-ANTIBODY AGAINST NEUROFILAMENT PROTEIN SPECIFICALLY LABELS A SUBPOPULATION OF RAT SENSORY NEURONS
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DOI：
10.1002/cne.902280211
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
ANDERTON, BH
中科院分区：
文献类型：
--
作者：
LAWSON, SN;HARPER, AA;ANDERTON, BH
一种针对神经丝蛋白的单克隆抗体（RT97）特异性和特异性地标记了大鼠背根神经节（DRG）神经元亚群。对于定量研究的7个神经节（L4和T13），标记细胞大小的频率分布直方图可以用单个正态分布拟合，其参数与该神经节内大光细胞群正态分布的参数非常接近。该抗体可作为大鼠DRG中大量轻神经元的特异性标记。这种抗体不能标记小的暗神经元群。在一个神经节中，对每个神经元是否被标记的主观分析直接与反应产物强度的微密度测量相比较。根据反应产物的强度绘制的神经元大小，标记的群体没有细分。除DRG外，在三叉神经节、迷走神经节、中脑V核中也发现了其他被该抗体强标记的神经元细胞体与未标记的小神经元相关，这些小神经元均由初级传入神经元组成，全部或部分来源于神经嵴。交感神经系统和中枢神经系统神经元细胞体未被标记或偶尔被标记得很轻，尽管免疫反应纤维在中枢神经系统中大量存在。
A monoclonal antibody (RT97) against neurofilament protein specifically and exclusively labeled a subpopulation of rat dorsal root ganglion (DRG) neurons. For 7 ganglia (L4 and T13) studied quantitatively, the frequency distribution histograms of the size of labeled cells could be fitted by a single normal distribution, whose parameters were extremely close to those of the normally distributed large light cell population in that ganglion. This antibody can be used as a much needed specific label for the large light population of neurons in rat DRG. The small dark neuron population was not labeled by this antibody. In one ganglion, the subjective analysis of whether each neuron was labeled or not was directly compared with microdensitometric measurements of reaction product intensity. No subdivisions of the labeled population were apparent on the basis of neuronal size plotted against intensity of the reaction product. Other neuronal cell bodies strongly labeled by this antibody were found in association with small unlabeled neurons, not only in DRG, but also in the trigeminal ganglion, the vagal ganglia, and the mesencephalic V nucleus, all of which are made up of primary afferent neurons and all of which are completely or partially derived from the neural crest. Sympathetic nervous system and CNS neuronal cell bodies were unlabeled or occasionally very lightly labeled, although immunoreactive fibers abound in the CNS.