Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose
Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose
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DOI：
10.1039/c5gc02660a
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发表时间：
2016-01-01
期刊：
影响因子：
9.8
通讯作者：
Hailes, Helen C.
中科院分区：
文献类型：
--
作者：
Subrizi, Fabiana;Cardenas-Fernandez, Max;Hailes, Helen C.
利用生物催化转化生物质很可能成为一项对未来生产化学积木、材料和运输燃料做出重大贡献的技术。本文研究了以甜菜浆中碳水化合物的主要成分L阿拉伯糖为原料，以简明、可持续的方式合成附加值化学品。利用转酮醇酶变体的生物催化转化已经被开发出来，用于从L-阿拉伯糖高效、可扩展地合成罕见的自然存在的酮庚糖--L-葡萄糖-七酮果糖。新的活性大肠杆菌TK突变体很容易接受L-阿拉伯糖，用多功能比色筛选试验鉴定，并在制备性规模上进行反应。
Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.