Substrate Specificity of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
Substrate Specificity of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
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荚膜醋杆菌 ATCC 11894 中糊精葡聚糖酶的底物特异性
DOI：
10.1271/bbb.58.330
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
S. Okada
中科院分区：
文献类型：
--
作者：
Kazuya Yamamoto;K. Yoshikawa;S. Okada
研究了糊精葡聚糖酶（EC 2.4.1.2；DDase）的底物特异性。该酶除淀粉和糊精外还作用于麦芽糖和异麦芽糖，但不作用于其他葡萄糖二糖。当各种糖类与作为葡萄糖基受体的水杨苷反应时，在与作为葡萄糖基供体的麦芽糖、异麦芽糖、淀粉和葡聚糖反应时，葡萄糖基残基被转移到水杨苷上。另一方面，当作为葡糖基供体的淀粉与各种糖反应时，淀粉的葡糖基残基被转移到非还原末端具有葡糖基或木糖基残基的d-葡萄糖、d-木糖和寡糖。甲基α-和β-d-葡萄糖苷也充当受体。此外，DDase 将葡萄糖残基从淀粉转移到葡萄糖衍生物，例如 2-脱氧-、2-乙酰氨基-2-脱氧-、3-O-甲基-和 6-脱氧-d-葡萄糖。当使用淀粉作为葡萄糖基供体时，通过转葡萄糖基化为d-葡萄糖作为受体形成的两种产物被发现是错误的...
The substrate specificity of dextrin dextranase (EC 2.4.1.2; DDase) was investigated. This enzyme acted on maltose and isomaltose in addition to starch and dextrin, but did not act on other gluco-disaccharides. When various saccharides were allowed to react with salicin as a glucosyl acceptor, glucosyl residues were transferred to salicin on the reaction with maltose, isomaltose, starch, and dextran as glucosyl donors. On the other hand, when starch as a glucosyl donor was allowed to react with various saccharides, glucosyl residues of starch were transferred to d-glucose, d-xylose, and oligosaccharides that had glucosyl or xylosyl residues at non-reducing termini. Methyl α- and β-d-glucosides also acted as acceptors. Furthermore DDase transferred glucosyl residues from starch to glucose derivatives such as 2-deoxy-, 2-acetamido-2-deoxy-, 3-O-methyl-, and 6-deoxy-d-glucoses. When starch was used as a glucosyl donor, two products formed by transglucosylation to d-glucose as an acceptor were found to be mal...