Insertion preference of maize and rice miniature inverted repeat transposable elements as revealed by the analysis of nested elements
Insertion preference of maize and rice miniature inverted repeat transposable elements as revealed by the analysis of nested elements
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DOI：
10.1105/tpc.13.11.2553
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发表时间：
2001-11-01
期刊：
影响因子：
11.6
通讯作者：
Wessler, SR
中科院分区：
文献类型：
--
作者：
Jiang, N;Wessler, SR
在玉米wax - b2等位基因中插入128 bp，发现了一个微型倒置重复转座元件（MITEs）家族。作为一类特殊的非自主元件，螨虫具有拷贝数高、体积小、与植物基因联系密切等特点。在玉米中，一些Tourist元素（命名为Tourist- zm）以相邻或嵌套插入的形式出现。为了确定多聚体的形成是否是螨虫的共同特征，我们进行了更彻底的调查，包括对多聚体比例的估计，使用了30.2 Mb的公开水稻基因组序列。在鉴定的6600个螨虫中，约有10%为多聚体。不同螨类的多聚体比例不同。在一些螨家族中，发现了高频率的自我插入。所有340个多路定时器都是唯一的这一事实表明，这些多路定时器不能进一步放大。
A 128-bp insertion into the maize waxy-B2 allele led to the discovery of Tourist, a family of miniature inverted repeat transposable elements (MITEs). As a special category of nonautonomous elements, MITEs are distinguished by their high copy number, small size, and close association with plant genes. In maize, some Tourist elements (named Tourist-Zm) are present as adjacent or nested insertions. To determine whether the formation of multimers is a common feature of MITEs, we performed a more thorough survey, including an estimation of the proportion of multimers, with 30.2 Mb of publicly available rice genome sequence. Among the 6600 MITEs identified, > 10% were present as multimers. The proportion of multimers differs for different MITE families. For some MITE families, a high frequency of self-insertions was found. The fact that all 340 multimers are unique indicates that the multimers are not capable of further amplification.