BODO-CAUDATUS MEDRNA AND 5S-RIBOSOMAL-RNA GENES - TANDEM ARRANGEMENT AND PHYLOGENETIC ANALYSES
BODO-CAUDATUS MEDRNA AND 5S-RIBOSOMAL-RNA GENES - TANDEM ARRANGEMENT AND PHYLOGENETIC ANALYSES
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DOI：
10.1016/0006-291x(92)91838-h
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发表时间：
1992-02-14
影响因子：
3.1
通讯作者：
CAMPBELL, DA
中科院分区：
文献类型：
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作者：
CAMPBELL, DA
PCR 扩增后已分离出 Bodo caudatusmini 外显子衍生的 RNA 基因重复序列。迷你外显子的 DNA 序列符合锥虫门迷你外显子共有序列，支持将博多包含在该组中。 TheB. caudatusmini-exon 重复序列还包含 5S 核糖体 RNA 基因，这是在锥虫 T 中发现的一种组织。 Rangeli和线虫的五个属。对微型外显子衍生的 RNA 基因和 5S 基因序列的系统发育分析表明，free-livingB.有尾虫与单基因锥虫的关系比与双基因锥虫的关系更密切。在这些比较期间还注意到眼虫转剪前导序列和锥虫微型外显子序列之间的相似性。
TheBodo caudatusmini-exon-derived RNA gene repeat has been isolated following PCR amplification. The DNA sequence of the mini-exon fits the trypanosomatid mini-exon consensus, supporting inclusion ofBodoin this group. TheB. caudatusmini-exon repeat also contains the 5S ribosomal RNA gene, an organization found in the trypanosomeT. rangeliand five genera of nematodes. Phylogenetic analysis of both mini-exon-derived RNA gene and 5S gene sequences show that the free-livingB. caudatusis more closely related to the monogeneticCrithidiathan the digeneticTrypanosoma. Similarity between theEuglena gracilis trans-spliced leader and trypanosomatid mini-exon sequences was also noted during these comparisons.