Sequencing PCR DNA amplified directly from a bacterial colony.
Sequencing PCR DNA amplified directly from a bacterial colony.
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DOI：
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发表时间：
1991
期刊：
影响因子：
2.7
通讯作者：
M. Hofmann;David A. Brian
中科院分区：
文献类型：
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作者：
M. Hofmann;David A. Brian
我们表明，直接从细菌菌落中不对称扩增的PCR产物可以被测序，以产生与将纯化的模板DNA用于PCR扩增步骤时获得的结果一样好的结果。使用任一模板，可以从典型的测序反应读取大于300个核苷酸。Taq DNA聚合酶用于PCR扩增和测序反应。
We show that PCR product asymmetrically amplified directly from a bacterial colony can be sequenced to yield results as good as those obtained when purified template DNA is used for the PCR amplification step. With either template, greater than 300 nucleotides can be read from a typical sequencing reaction. Taq DNA polymerase was used for both the PCR amplification and sequencing reactions.