A dominant mutation of TWISTED DWARF 1 encoding an α-tubulin protein causes severe dwarfism and right helical growth in rice
A dominant mutation of TWISTED DWARF 1 encoding an α-tubulin protein causes severe dwarfism and right helical growth in rice
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DOI：
10.1266/ggs.84.209
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发表时间：
2009-06-01
影响因子：
1.1
通讯作者：
Kitano, Hidemi
中科院分区：
文献类型：
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作者：
Sunohara, Hidehiko;Kawai, Takayuki;Kitano, Hidemi
矮秆是许多植物物种中常见的突变类型。通过对水稻、拟南芥、豌豆和玉米等植物矮秆突变体的分析，阐明了几种植物生长调节物质生物合成和信号转导的途径和调控因子。然而，控制矮秆的遗传机制还没有得到很好的表征，大多数矮秆突变体的致病基因仍然没有被发现。在这里，我们报告了一个显性突变体，扭曲矮1-1（Tid 1 -1），表现出矮秆和扭曲生长的水稻。Tid 1 -1表现出叶片和茎的正螺旋生长和根的缩短。它们还显示了茎顶端分生组织中细胞数量的增加。Tid 1 -1叶片中的细胞通常形状不佳，可能是由于不规则的细胞分裂。根中细胞的伸长在伸长区被抑制，并且根顶端分生组织中的细胞被扩大。TID 1的图位克隆揭示了它编码包含微管的α-微管蛋白，并且是拟南芥LEFTY基因的直系同源物。我们的Tid 1 -1突变体的分析表明，微管的动态不仅影响双子叶植物和单子叶植物的各向异性生长，而且分生组织的活动和总植物形态。
Dwarfism is a common type of mutation in many plant species. The pathways and factors regulating biosynthesis and signaling of several plant growth regulators have been clarified through analyses of dwarf mutants in rice, Arabidopsis, pea, and maize. However, the genetic mechanisms controlling dwarfism are not well characterized, and the causal genes underlying most dwarf mutants are still uncovered. Here, we report a dominant mutant, Twisted dwarf 1-1 (Tid1-1), showing dwarfism and twisted growth in rice. Tid1-1 exhibit right helical growth of the leaves and stem and shortening of the roots. They also show an increased number of cells in the shoot apical meristem. Cells in the leaves of Tid1-1 are often ill-shapen, possibly owing to irregular cell division. Cell elongation in roots is suppressed in the elongation zone, and cells in the root apical meristem are enlarged. Map-based cloning of TID1 revealed that it encodes an a-tubulin protein comprising microtubules and is an ortholog of Arabidopsis LEFTY genes. Our analysis of the Tid1-1 mutant revealed that the dynamics of microtubules affects not only anisotropic growth in both dicots and monocots, but also meristematic activity and gross plant morphology.