Quantitative Monitoring of Rutin in Human Urine by Flow Injection-Chemiluminescence Analysis
Quantitative Monitoring of Rutin in Human Urine by Flow Injection-Chemiluminescence Analysis
复制标题
流动注射-化学发光分析法定量监测人尿液中的芦丁
DOI：
10.1002/jccs.201600884
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发表时间：
2017
影响因子：
1.8
通讯作者：
Song Zhenghua
中科院分区：
文献类型：
--
作者：
Xin Ladi;Li Yajuan;Wu Lingmin;Zhao Jingchan;Song Zhenghua
本文基于芦丁对发光-胰凝乳酶（CT）体系化学发光强度的抑制作用，建立了一种快速、灵敏的流动注射化学发光（FI - CL）定量测定芦丁的方法。在0.1 ~ 30.0 ng/mL范围内，与芦丁浓度的对数成正比。建立了芦丁的定量分析方法，检测限为0.03 ng/mL （3σ）。在2.0 mL/min的流速下，从取样到洗涤，整个分析过程可在30 s内完成，样品效率为120 h−1。该方法可用于人口服尿液中芦丁的测定，回收率为93.9 ~ 108.1%，相对标准偏差<4.0% （n= 5）。结果表明，两名志愿者在~2.5 h时尿浓度达到最大值，8 h总排泄率分别为（83.5±0.6）%和（86.8±0.7）%。获得了半衰期（1.05±0.02 h）、吸收速率常数（1.18±0.01 h−1）、消除速率常数（0.70±0.01 h−1）等药动学参数。通过FI - CL、荧光和分子对接等方法探讨了鲁米诺- ct -芦丁反应可能的CL机制。
In this paper, a rapid and sensitive flow injection‐chemiluminescence (FI‐CL) method is proposed for the quantitative determination of rutin based on the inhibitory effect of rutin on the chemiluminescence intensity from the luminol–chymotrypsin (CT) system. The decrease of CL intensity was found to be proportional to the logarithm of rutin concentration in the range 0.1–30.0 ng/mL. A method for the quantification of rutin is proposed, with the limit of detection (LOD) of 0.03 ng/mL (3σ). A complete analytical process including sampling and washing for rutin determination, which was conducted at a flow rate of 2.0 mL/min, could be performed completely within 30 s, yielding a sample efficiency of 120 h−1. The proposed procedure was successfully applied for the determination of rutin in human urine after oral intake, with recoveries varying from 93.9 to 108.1% and relative standard derivation <4.0% (n= 5). Results showed that urine reached the maximum concentration at ~2.5 h, and the total excretion ratios were (83.5 ± 0.6) and (86.8 ± 0.7)%, respectively, for two volunteers in 8 h. The pharmacokinetic parameters, including the half‐life (1.05 ± 0.02 h), absorption rate constant (1.18 ± 0.01 h−1), and elimination rate constant (0.70 ± 0.01 h−1), were obtained. The possible CL mechanism of the luminol–CT–rutin reaction is discussed by FI‐CL, fluorescence, and molecular docking methods.