Genetic instability and apoptotic cell death during neoplastic progression of v-myc-initiated B-cell lymphomas in the bursa of Fabricius.
Genetic instability and apoptotic cell death during neoplastic progression of v-myc-initiated B-cell lymphomas in the bursa of Fabricius.
复制标题
法氏囊中 v-myc 引发的 B 细胞淋巴瘤肿瘤进展过程中的遗传不稳定性和细胞凋亡。
DOI：
10.1101/sqb.1994.059.01.056
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Loring,G
中科院分区：
文献类型：
--
作者：
Neiman,PE;Summers,J;Thomas,SJ;Xuereb,S;Loring,G
METHODSBursal transplantation assay. The basic technique used for these studies was reconstitution of cyclophosphamide-ablated bursal follicle populations with donor cells transduced with a retrovirus expressing a v-myc oncogene. The methodology for this approach has been described previously (Neiman et al. 1985; Thompson et al. 1987) and is shown schematically in Figure 1:(1) Recipient chick embryos between 18 days of embryogenesis and day-of-hatch were treated with cyclophosphamide to selectively destroy the bursal lymphoid population (Eskola and Toivanen 1974).(2) Bursal follicles from syngeneic 18-day chick embryo donors were dispersed and co-cultivated for 8-16 hours in the presence of 100 ng/ml phorbol dibutyrate to inhibit cell death (Ewart and Weber 1987; Asakawa et al. 1993) on adherent monolayers producing the v-myc-transducing retrovirus and injected intravenously into the cyclophosphamide-prepared recipients.(3) Stem cells in the donor population reconstitute the ablated follicles. Those successfully transduced and expressing v-n~ J Primary Transplant
