Portable Sensor for the Detection of Choline and Its Derivatives Based on Silica Isoporous Membrane and Gellified Nanointerfaces
Portable Sensor for the Detection of Choline and Its Derivatives Based on Silica Isoporous Membrane and Gellified Nanointerfaces
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基于二氧化硅等孔膜和凝胶化纳米界面的便携式胆碱及其衍生物检测传感器
DOI：
10.1021/acssensors.7b00166
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发表时间：
2017-06-01
期刊：
影响因子：
8.9
通讯作者：
Su, Bin
中科院分区：
文献类型：
--
作者：
Xie, Lisiqi;Huang, Xiao;Su, Bin
将二氧化硅等孔膜（SIM）和聚氯乙烯- 1,2-二氯乙烷有机凝胶（PVC-DCE）集成在3d打印聚合物芯片上，制备了便携式安培离子传感器。通过在传感器中加入一微升的样品液滴，并通过识别由SIM模板化的水/有机凝胶界面阵列上的离子转移电位和电流大小，可以完成水样品中离子种类的检测。由于SIM的超小通道尺寸（直径2-3 nm），高通道密度（4 × 108 μ m(-2)）和超薄厚度（80 nm），纳米级水/有机凝胶（纳米w /凝胶）界面阵列表现为具有两个背对背半球形质量扩散区的微界面。因此，通过纳米w /凝胶界面阵列的非均相离子转移产生稳态s型电流波。用该便携式传感器检测了水溶液中胆碱（Ch）及其衍生物，包括乙酰胆碱（ACh）、苯甲酰胆碱（BCh）和阿托品（AP）。利用差分脉冲溶出伏安法（DPSV），实现了这些分析物的定量，检测限（LOD）低至1 μ m。此外，由于SIM基于尺寸/电荷的选择性和防污能力，便携式离子传感器对体内可能共存的各种潜在干扰不敏感。有了这个优先级，便携式离子传感器能够定量测定稀释尿液和血液样本中的Ch及其衍生物。尿液中Ch、ACh、AP和BCh的lod分别为1.12、1.30、1.08和0.99 μ M，血液中Ch、ACh、AP和BCh的lod分别为3.61、3.38、2.32和1.81 μ M。
A portable amperometric ion sensor was fabricated by integrating silica isoporous membrane (SIM) and organogel composed of polyvinyl chloride and 1,2-dichloroethane (PVC-DCE) on a 3D-printed polymer chip. The detection of ionic species in aqueous samples could be accomplished by adding a microliter of sample droplet to the sensor and by identifying the ion-transfer potential and current magnitude at the water/ organogel interface array templated by SIM. Thanks to the ultrasmall channel size (2-3 nm in diameter), high channel density (4 x 108 mu m(-2)), and ultrathin thickness (80 nm) of SIM, the ensemble of nanoscopic water/ organogel (nano-W/Gel) interface array behaved like a microinterface with two back-to-back hemispherical mass diffusion zones. So, the heterogeneous ion-transfer across the nano-W/Gel interface array generated a steady-state sigmoidal current wave. The detection of choline (Ch) and its derivatives, including acetylcholine (ACh), benzoylcholine (BCh), and atropine (AP), in aqueous samples was examined with this portable sensor. Using differential pulse stripping voltammetry (DPSV), the quantification of these analytes was achieved with a limit of detection (LOD) down to 1 mu M. Moreover, the portable ion sensor was insensitive to various potential interferents that might coexist in vivo, owing to size-/ charge-based selectivity and antifouling capacity of SIM. With this priority, the portable ion sensor was able to quantitatively determine Ch and its derivatives in diluted urine and blood samples. The LODs for Ch, ACh, AP, and BCh in urine were 1.12, 1.30, 1.08, and 0.99 mu M, and those for blood samples were 3.61, 3.38, 2.32, and 1.81 mu M, respectively.