Reorganization of longitudinal compartments in the laterally protruding paraflocculus of the postnatal mouse cerebellum.
Reorganization of longitudinal compartments in the laterally protruding paraflocculus of the postnatal mouse cerebellum.
复制标题
出生后小鼠小脑横向突出的副絮球纵向区室的重组。
DOI：
10.1002/cne.24849
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sugihara I.
中科院分区：
文献类型：
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作者：
Panezai SK;Luo Y;Vibulyaseck S;Sarpong GA;Nguyen-Minh VT;Nedelescu H;Hirano S;Sugihara I.
旁小叶和相邻的小小叶在小鼠小脑的腹外侧形成显著的突起，其中纵向隔室明显垂直于矢状面。这些小叶的这种解剖排列的发育过程尚未完全阐明。在这里，我们使用pcdh 10-lacZ敲入（OL-KO）、IP 3R 1-nls-lacZ转基因（1 NM 13）和Gpr 26 cre-Ai 9-AldocV小鼠的遗传易处理性来跟踪隔室的发育，并检查了副绒球和绒球内浦肯野细胞的局部纵向方向。我们在胚胎OL-KO小脑中观察到一个明显的pcdh 10阳性（pcdh 10+）室，而副小叶和其他小叶有一个连续的paravermalpcdh 10+室。在出生后的第一周，paraflocellarpcdh 10+室横向转移到最外侧边缘的尾部突出paraflocellus。虽然paraflocellarpcdh 10+室的最内侧边缘仍然在paraflocellus的nonprojectile部分，它是从原来的continuouspcdh 10+室在copula dogs中断。在成人小脑中，侧连的旁小叶的局部纵向方向随着连接索的中外侧范围逐渐沿着改变，几乎变得垂直于矢状面。这种方向上的旋转变化来自于短的U形胚胎小脑，其中小叶和副小叶的表面是侧向定向的。这些结果表明，副绒球的特殊隔室组织起源于胚胎共同的半球隔室组织，并在出生后第一周的重大重组过程中形成。
The paraflocculus and the neighboring smaller flocculus form a remarkable protrusion in the ventrolateral aspect of the mouse cerebellum, in which the longitudinal compartments are conspicuously oriented perpendicularly to the sagittal plane. The developmental process of such anatomical arrangements in these lobules has not been fully clarified. Here, we used the genetic tractability ofpcdh10‐lacZknock‐in (OL‐KO),IP3R1‐nls‐lacZtransgenic (1NM13) and Gpr26cre‐Ai9‐AldocV mice to track the development of compartments and examined local longitudinal orientation of Purkinje cells within the paraflocculus and flocculus. We observed a distinctpcdh10‐positive (pcdh10+) compartment in the flocculus, whereas the paraflocculus and other lobules had a continuous paravermalpcdh10+ compartment, in the embryonic OL‐KO cerebellum. During the first postnatal week, the parafloccularpcdh10+ compartment shifted laterally to the most lateral edge in the caudal part of the protruding paraflocculus. Although the most medial edge of the parafloccularpcdh10+ compartment remained in the nonprotruding part of the paraflocculus, it was disrupted from the originally continuouspcdh10+ compartment in the copula pyramidis. The local longitudinal orientation changed gradually along with the mediolateral extent of the copula pyramidis, almost becoming perpendicular to the sagittal plane in the laterally connected paraflocculus in the adult cerebellum. This rotational change in orientation was derived from the short U‐shaped embryonic cerebellum, in which the surfaces of the flocculus and paraflocculus were oriented laterally. These results indicated that the peculiar compartmental organization of the paraflocculus originates from the embryonic common hemispheric compartmental organization and shaped by the significant reorganization process in the first postnatal week.