Study on cellular events in post-thymectomy autoimmune oophoritis in mice. II. Requirement of Lyt-1 cells in normal female mice for the prevention of oophoritis.
Study on cellular events in post-thymectomy autoimmune oophoritis in mice. II. Requirement of Lyt-1 cells in normal female mice for the prevention of oophoritis.
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DOI：
10.1084/jem.156.6.1577
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发表时间：
1982-12-01
期刊：
影响因子：
--
通讯作者：
Nishizuka Y
中科院分区：
文献类型：
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作者：
Sakaguchi S;Takahashi T;Nishizuka Y
通过单次腹腔注射来自正常成年雌性小鼠的脾细胞或胸腺细胞，预防了A/J小鼠在胸腺切除术（NTx）后发生的自身免疫性卵巢炎。Tx后2周内给予脾细胞可预防卵巢炎。当从卵巢切除的小鼠中获得脾细胞时，需要四倍多的细胞来预防卵巢炎，但出生后第7天切除卵巢的小鼠的脾细胞与正常雌性小鼠的脾细胞预防卵巢炎的能力相当。正常A/J小鼠的脾细胞能阻止NTx A/J小鼠卵巢炎的发生，它们是Thy-1+、Lyt-1+、23-、Ia-、Qa-1-，对400 rad体外照射敏感，对环磷酰胺或抗胸腺细胞血清有抵抗力，成年胸腺切除术也不能清除。具有抗卵巢炎能力的胸腺细胞也被发现为Lyt-1+，23-和TL-1，2，3-。这些结果似乎与NTx小鼠中Lyt-1亚群显著减少的发现密切相关。结果表明，在这种胸腺切除术后自身免疫性卵巢炎中，NTx废除了Lyt-1 T细胞亚群，该细胞亚群在针对卵巢抗原的自身反应细胞的发展中起抑制或调节作用，并最终可能导致自身免疫性卵巢炎。
Autoimmune oophoritis that develops in A/J mice after neonatally thymectomy (NTx) was prevented by a single intraperitoneal injection of spleen cells or thymocytes from normal adult female mice. Prevention of oophoritis was achieved when spleen cells were given within 2 wk after Tx. When spleen cells were obtained from neonatally oophorectomized mice, four times more cells were required for the prevention of oophoritis, but those from the mice oophorectomized on day 7 after birth had equivalent capacity to prevent oophoritis to those from normal female mice. The spleen cells from normal A/J mice that prevented the development of oophoritis in NTx A/J mice were Thy-1+, Lyt-1+,23-, Ia-, Qa-1-, sensitive to in vitro irradiation with 400 rad, resistant to administration of cyclophosphamide or anti-thymocyte serum, and were not eliminated by adult thymectomy. Thymocytes with oophoritis-preventing capacity were also found to be Lyt-1+,23- and TL- 1,2,3-. These results seem to correlate well with the finding that the Lyt-1 subpopulation is substantially decreased in NTx mice. The results suggest that, in this post-thymectomy autoimmune oophoritis, NTx abrogates the Lyt-1 T cell subpopulation that serves as suppressive or regulatory cells over developing self-reactive cells directed toward ovarian antigens, and eventually may cause autoimmune oophoritis.