Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia.
Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia.
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DOI：
10.1007/978-3-319-17641-3_5
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发表时间：
2015
影响因子：
--
通讯作者：
Hill ES
中科院分区：
文献类型：
--
作者：
Frost WN;Brandon CJ;Bruno AM;Humphries MD;Moore-Kochlacs C;Sejnowski TJ;Wang J;Hill ES
使用快速电压敏感染料的光学记录使得在适当的准备下，同时监测大量单个神经元的动作电位成为可能。在这里，我们描述了这样做的方法，包括考虑不同的染料和成像系统，将光学信号与它们的源神经元相关的方法，获得良好信号的程序，以及使用独立分量分析将原始光学数据分选成单个神经元轨迹。这些组合工具代表了一种强大的方法，用于以高时间和空间分辨率大规模记录神经网络。
Optical recording with fast voltage sensitive dyes makes it possible, in suitable preparations, to simultaneously monitor the action potentials of large numbers of individual neurons. Here we describe methods for doing this, including considerations of different dyes and imaging systems, methods for correlating the optical signals with their source neurons, procedures for getting good signals, and the use of Independent Component Analysis for spike-sorting raw optical data into single neuron traces. These combined tools represent a powerful approach for large-scale recording of neural networks with high temporal and spatial resolution.