Bacterial community structure and effects of picornavirus infection on the anterior nares microbiome in early childhood
Bacterial community structure and effects of picornavirus infection on the anterior nares microbiome in early childhood
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DOI：
10.1186/s12866-018-1372-8
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发表时间：
2019-01-07
期刊：
影响因子：
4.2
通讯作者：
Mikolajczyk, Rafael T.
中科院分区：
文献类型：
--
作者：
Caputo, Mahrrouz;Zoch-Lesniak, Beate;Mikolajczyk, Rafael T.
背景关于儿童早期鼻腔微生物组以及呼吸道感染对婴儿鼻腔微生物组成的影响知之甚少。在这里，我们调查的时间动态和多样性的细菌组成的前鼻孔在儿童参加daycare centers.ResultsFor我们的调查，我们认为76父母采取的鼻拭子的26名儿童（年龄13至36个月）收集在一个为期3个月的研究期间。总体而言，鼻腔细菌群落结构没有明显的年龄特异性效应或季节性变化。在14个健康儿童的子样本中，个体类群的相对丰度以及整体多样性没有显示出相关的变化，表明在整个研究期间，群落结构稳定。此外，鼻细菌谱聚类受试者特异性，与受试者间计算相比，受试者内计算中的Bray-Curtis相似性升高。其余12名儿童提供了在小核糖核酸病毒感染（PVI）期间以及PVI之前或之后采集的样本。当比较（i）肺静脉隔离期间采集的样本与14名健康儿童的样本以及（ii）肺静脉隔离期间采集的样本与同一个体肺静脉隔离后采集的样本时，我们检测到链球菌属成员的相对丰度与肺静脉之间存在关联。此外，PVI期间的多样性高于infection.ConclusionsOur研究结果表明，个性化结构的鼻腔细菌群落已经建立在幼儿期，可以检测到超过3个月的时间范围。对婴儿进行更长时间的频繁拭子采样研究，可以调查细菌群落的某些参数（如时间变异性）是否与病毒感染有关。
BackgroundLittle is known regarding the nasal microbiome in early childhood and the impact of respiratory infection on the infants' nasal microbial composition. Here we investigated the temporal dynamics and diversity of the bacterial composition in the anterior nares in children attending daycare centers.ResultsFor our investigation, we considered 76 parental-taken nasal swabs of 26 children (aged 13 to 36months) collected over a study period of 3 months. Overall, there was no significant age-specific effect or seasonal shift in the nasal bacterial community structure. In a sub-sample of 14 healthy children the relative abundance of individual taxa as well as the overall diversity did not reveal relevant changes, indicating a stable community structure over the entire study period. Moreover, the nasal bacterial profiles clustered subject-specific with Bray-Curtis similarities being elevated in intra-subject calculations compared to between-subject calculations. The remaining subset of 12 children provided samples taken during picornavirus infection (PVI) and either before or after a PVI. We detected an association between the relative abundance of members of the genus Streptococcus and PV when comparing both (i) samples taken during PVI with samples out of 14 healthy children and (ii) samples taken during PVI with samples taken after PVI within the same individual. In addition, the diversity was higher during PVI than after infection.ConclusionsOur findings suggest that a personalized structure of the nasal bacterial community is established already in early childhood and could be detected over a timeframe of 3 months. Studies following infants over a longer time with frequent swab sampling would allow investigating whether certain parameter of the bacterial community, such as the temporal variability, could be related to viral infection.