Inactivation of SARS-CoV-2 by Catechins from Green Tea
Inactivation of SARS-CoV-2 by Catechins from Green Tea
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DOI：
10.7883/yoken.jjid.2020.902
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发表时间：
2021-09-01
影响因子：
2.2
通讯作者：
Oshitani, Hitoshi
中科院分区：
文献类型：
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作者：
Nishimura, Hidekazu;Okamoto, Michiko;Oshitani, Hitoshi
绿色茶提取物在体外以剂量依赖方式有效灭活严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。将绿色茶儿茶素混合试剂10倍稀释液与病毒培养液按9：1的体积比混合，室温下孵育5 min，10 mg/mL儿茶素试剂溶液使病毒滴度降低4.2 log，1.0 mg/mL儿茶素试剂溶液使病毒滴度降低1 log。单独使用试剂对细胞进行感染前处理不会影响病毒生长。此外，使用WST-8系统测定仅用试剂处理的细胞的宿主细胞活力，几乎没有观察到处理对宿主细胞的损伤。这些结果表明，在接种前用试剂直接处理病毒降低了病毒活性，儿茶素可能具有抑制SARS-CoV-2感染的潜力。
Green tea extracts effectively inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro in a dose-dependent manner. Ten-fold serially diluted solutions of catechin mixture reagent from green tea were mixed with the viral culture fluid at a volume ratio of 9:1, respectively, and incubated at room temperature for 5 min. The solution of 10 mg/mL catechin reagent reduced the viral titer by 4.2 log and 1.0 mg/mL solution by one log. Pre-infection treatment of cells with the reagent alone did not affect viral growth. In addition, cells treated with only the reagent were assayed for host cell viability using the WST-8 system, and almost no host cell damage by the treatment was observed. These findings suggested that the direct treatment of virus with the reagent before inoculation decreased the viral activity and that catechins might have the potential to suppress SARS-CoV-2 infection.