Long-term seasonal and interannual variability of marine aerobic anoxygenic photoheterotrophic bacteria
Long-term seasonal and interannual variability of marine aerobic anoxygenic photoheterotrophic bacteria
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DOI：
10.1038/s41396-019-0401-4
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发表时间：
2019-08-01
期刊：
影响因子：
11
通讯作者：
Ferrera, Isabel
中科院分区：
文献类型：
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作者：
Auladell, Adria;Sanchez, Pablo;Ferrera, Isabel
我们利用Blanes Bay微生物观测站的高通量pufM基因测序结合多变量、时间序列和共生分析，研究了好氧产氧光营养细菌(AAP细菌)的长期时间动态。AAP细菌是沿海海洋微生物食物网中的一个相关功能群。此外，利用元基因组学，我们测试了所使用的引物是否准确地捕捉到了最相关的AAP组的季节性。各季节群落结构的贡献率最大的是K类群(GammaProtebacteria)，其中E类群和G类群(Alphaprobacteria)在春季较多。多样性指数表现出明显的季节变化趋势，冬季最大，与AAP丰度相反。多变量分析显示，样本按季节聚集，相关比例的差异由日长、温度、盐度、光营养纳米鞭毛虫丰度、叶绿素a和硅酸盐浓度解释。时间序列分析显示出强烈的共生节律模式，但在相同的系统群中，甚至在符合相同操作分类单位(OTU)的不同扩增子序列变体(ASV)中，存在明显的季节性行为。总而言之，我们的结果描绘了AAP组合为高度季节性和重复的，但包含了显示出独特的时间生态位划分的生态型，而不是一个具有凝聚力的功能群。
We studied the long-term temporal dynamics of the aerobic anoxygenic phototrophic (AAP) bacteria, a relevant functional group in the coastal marine microbial food web, using high-throughput sequencing of the pufM gene coupled with multivariate, time series and co-occurrence analyses at the Blanes Bay Microbial Observatory (NW Mediterranean). Additionally, using metagenomics, we tested whether the used primers captured accurately the seasonality of the most relevant AAP groups. Phylogroup K (Gammaproteobacteria) was the greatest contributor to community structure over all seasons, with phylogroups E and G (Alphaproteobacteria) being prevalent in spring. Diversity indices showed a clear seasonal trend, with maximum values in winter, which was inverse to that of AAP abundance. Multivariate analyses revealed sample clustering by season, with a relevant proportion of the variance explained by day length, temperature, salinity, phototrophic nanoflagellate abundance, chlorophyll a, and silicate concentration. Time series analysis showed robust rhythmic patterns of co-occurrence, but distinct seasonal behaviors within the same phylogroup, and even within different amplicon sequence variants (ASVs) conforming the same operational taxonomic unit (OTU). Altogether, our results picture the AAP assemblage as highly seasonal and recurrent but containing ecotypes showing distinctive temporal niche partitioning, rather than being a cohesive functional group.