Suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in Nilaparvata lugens Stal
Suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in Nilaparvata lugens Stal
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DOI：
10.1038/srep28111
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发表时间：
2016-06-16
影响因子：
4.6
通讯作者：
Wu, Jin-Cai
中科院分区：
文献类型：
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作者：
Ge, Lin-Quan;Xia, Ting;Wu, Jin-Cai
在我们之前对褐飞虱（BPH）、褐飞虱（Nilaparvata lugens）的研究中，与未处理的对照组相比，三唑磷（tzp）处理导致雄性精子发生相关蛋白5样基因（NlSPATA5）的显著上调。与未经处理的雄性交配的雌性相比，与经tzp处理的雄性交配显著增加了繁殖力（以产卵数量计算）。由于SPATA5在哺乳动物精子发育中起作用，并在睾丸中表达，我们提出了NlSPATA5在BPH精液中发生的假设，并通过交配在生殖力中起作用。我们通过研究抑制BPH雄性NlSPATA5表达对繁殖力的影响来验证这一假设。与对照组相比，NlSPATA5表达降低导致雄性副腺蛋白含量和生殖系统发育下降。雄性的这些变化导致雌性的产卵前期延长和繁殖力下降。对于两种性别，我们在体重、产卵期和寿命方面与对照组相比没有记录差异。NlSPATA5在雄性中的抑制也导致其交配对象脂肪体和卵巢蛋白含量、酵母样共生体丰度和卵巢发育以及卵黄原蛋白基因表达的降低。我们推测NlSPATA5表达的增加可能是tzp驱动BPH繁殖和种群增加的分子机制之一。
In our previous study with the brown planthopper (BPH), Nilaparvata lugens, triazophos (tzp) treatments led to substantial up-regulation of a male spermatogenesis-associated protein 5-like gene (NlSPATA5) compared to untreated controls. Mating with tzp-treated males significantly increased fecundity (as numbers of eggs laid), relative to females mated with untreated males. Because SPATA5 acts in mammalian sperm development and is expressed in testes, we posed the hypothesis that NlSPATA5 occurs in BPH seminal fluid and it operates in fecundity via mating. We tested the hypothesis by investigating the influence of suppressing NlSPATA5 expression in BPH males on fecundity. Reduced expression of NlSPATA5 led to decreased male accessory gland protein content and reproductive system development compared to controls. These changes in males led to prolonged pre-oviposition periods and decreased fecundity in females. For both genders, we recorded no difference in the body weight, oviposition periods, and longevity compared to controls. NlSPATA5 suppression in males also led to decreased fat body and ovarian protein content, yeast-like symbionts abundance and ovarian development as well as vitellogenin gene expression in their mating partners. We infer that increased NlSPATA5 expression may be one molecular mechanism of tzp-driven reproduction and population increases in BPH.