Combination drug treatment prolongs survival of experimentally infected mice with silver-haired bat rabies virus.
Combination drug treatment prolongs survival of experimentally infected mice with silver-haired bat rabies virus.
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DOI：
10.1016/j.vaccine.2018.05.065
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发表时间：
2019-08
期刊：
影响因子：
5.5
通讯作者：
B. Martina;M. Smreczak;A. Orłowska;A. Marzec;P. Trębas;Jouke M. Roose;J. Żmudziński;I. Gerhauser;P. Wohlsein;W. Baumgärtner;A. Osterhaus;P. Koraka
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文献类型：
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作者：
B. Martina;M. Smreczak;A. Orłowska;A. Marzec;P. Trębas;Jouke M. Roose;J. Żmudziński;I. Gerhauser;P. Wohlsein;W. Baumgärtner;A. Osterhaus;P. Koraka
狂犬病是人类和动物的致命疾病，每年造成约6万人死亡。目前，除了暴露后预防（PEP）之外，没有可用的治疗方法，只要发生与狂犬病动物的接触，就可以进行这种治疗。在这里，我们描述了在感染后第4天开始的组合治疗的有益效果，包含抗病毒药物和免疫调节剂在感染小鼠中。联合治疗可显著延长存活时间（P < 0.05），并显著降低脑和脊髓中的病毒RNA（P < 0.05）。此外，治疗影响了通过TNF-α水平测量的焦亡和细胞凋亡以及早期炎症反应的标志物。狂犬病病毒感染的小鼠没有形态学病变，几乎没有炎症迹象。然而，这些在不同的组之间并不显著。
Rabies is a lethal disease in humans and animals, killing approximately 60,000 people every year. Currently, there is no treatment available, except post-exposure prophylaxis (PEP) that can be administered whenever exposure to a rabid animal took place. Here we describe the beneficial effects of a combination treatment initiated at day 4 post infection, containing anti-viral drugs and immune modulators in infected mice. Combination therapy resulted in significant increase in survival time (P < 0.05) and significantly lowers viral RNA in the brain and spinal cord (P < 0.05). Furthermore, treatment influenced markers of pyroptosis and apoptosis and early inflammatory response as measured by the levels of TNF-α. Morphological lesions were absent in rabies virus infected mice with few signs of inflammation. However, these were not significant between the different groups.