Approaches to Fabricating Multiple-Layered Vascular Scaffolds Using Hybrid Electrospinning and Thermally Induced Phase Separation Methods
Approaches to Fabricating Multiple-Layered Vascular Scaffolds Using Hybrid Electrospinning and Thermally Induced Phase Separation Methods
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DOI：
10.1021/acs.iecr.5b03462
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发表时间：
2016-02-03
影响因子：
4.2
通讯作者：
Turng, Lih-Sheng
中科院分区：
文献类型：
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作者：
Mi, Hao-Yang;Jing, Xin;Turng, Lih-Sheng
近年来，小直径血管支架的制备一直是一个挑战，尤其是多层支架。本研究提出了两种制备三层结构血管支架的方法，即静电纺丝法和热诱导相分离法。结果发现，静电纺丝纤维具有致密的纤维结构，提供良好的机械性能。多孔TIPS层具有高孔隙率和孔互连性，以促进细胞渗透;然而，单独的这种结构不能确保足够的机械性能用于手术应用。由静电纺TPU、TIPS TPU和静电纺PPC层组成的三层支架显示出最高的机械性能以及用于血管移植物应用的最佳结构和尺寸。初步的内皮细胞培养结果表明，细胞可以附着在支架的内表面上并在支架的内表面上增殖，存活率>95%。
Fabrication of small-diameter vascular scaffolds has been a challenge in recent years, especially scaffolds with multiple layers. In this study, two approaches were proposed to fabricate triple-layered vascular scaffolds based on the electrospinning method and the thermally induced phase separation (TIPS) method. It was found that the electrospun fibers had a compact fibrous structure that provided good mechanical properties. The porous TIPS layer had high porosity and pore interconnectivity to facilitate cell penetration; however, this structure alone could not ensure sufficient mechanical properties for surgical applications. The triple-layered scaffolds, which consisted of electrospun TPU, TIPS TPU, and electrospun PPC layers, showed the highest mechanical properties and best structure and dimensions for vascular graft applications. Preliminary endothelial cell culture results showed that the cells could attach to and proliferate on the inside surface of the scaffolds with >95% viability.