Infection of sheep and monkeys with Langat virus: cross-protection against other viruses of the Russian spring-summer complex
Infection of sheep and monkeys with Langat virus: cross-protection against other viruses of the Russian spring-summer complex
复制标题
绵羊和猴子感染冷加特病毒：针对俄罗斯春夏复合体其他病毒的交叉保护
作者：
C. E. G. Smith;D. Mcmahon
1. 绵羊对Langat病毒的易感性进行了研究。在绵羊皮下或脑内接种均未发现病毒血症或症状。2. 只有脑内接种106·0 ~ 108·0小鼠icld 50病毒的绵羊产生大量中和抗体。3. 三分之二的绵羊接种了不同剂量的Langat病毒后，经受住了loupingill病毒的皮下攻击和脑内淀粉的攻击。所有脑内接种的绵羊都在这种形式的攻击中存活下来，但无论是皮下接种还是脑内接种，都没有绵羊经受住louping病的脑内攻击。4. 在一项田间试验中，接种了Langat病毒并在Camlarg和Dalcairnie暴露于自然louping病感染的10头猪中有3头死于该病，而未接种疫苗的8头猪全部死于该病。在诺克格雷，没有猪感染该病，但93%的猪在转移到卡姆格雷后产生了该病抗体。脑内、皮下或口服感染Langat病毒的猴子表现为低滴度病毒血症，无临床症状或脑组织组织学改变，并产生高滴度抗体。接种了中欧蜱传脑炎或Kyasanur森林病病毒疫苗的猴子与出现疾病证据的对照猴子相比仍然健康。
1. The susceptibility of sheep to infection with Langat virus has been studied. No viraemia or symptoms were detected in sheep inoculated either subcutaneously or intracerebrally. 2. Only those sheep inoculated intracerebrally with 106·0–108·0 mouse IC LD 50 of virus developed significant quantities of neutralizing antibody. 3. Two-thirds of sheep vaccinated with varying doses of Langat virus withstood subcutaneous challenge of louping-ill virus followed by intracerebral starch. All the intracerebrally vaccinated sheep survived this form of challenge but no sheep, whether vaccinated subcutaneously or intracerebrally, withstood intracerebral challenge of louping ill. 4. In a field trial, three of ten hoggs vaccinated with Langat virus and exposed to natural louping ill infection at Camlarg and Dalcairnie died of the disease compared with all eight of the non-vaccinated hoggs. At Knockgray, there was no louping ill infection, but 93% of the hoggs from this hirsel developed louping ill antibody after transfer to Camlarg. 5. Monkeys infected intracerebrally, subcutaneously or orally with Langat virus showed a low titre viraemia without clinical symptoms or histological changes in the brain and developed high titres of antibody. Vaccinated monkeys challenged with either Central European tick-borne encephalitis or Kyasanur Forest disease viruses remained healthy compared with control monkeys which showed evidence of disease.