Genome-wide identification and analysis of AP2/ERF transcription factors related to camptothecin biosynthesis in Camptotheca acuminata
Genome-wide identification and analysis of AP2/ERF transcription factors related to camptothecin biosynthesis in Camptotheca acuminata
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DOI：
10.1016/s1875-5364(20)30070-4
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发表时间：
2020-08-01
影响因子：
4.6
通讯作者：
Song Jing-Yuan
中科院分区：
文献类型：
--
作者：
Hu Ya-Ting;Xu Zhi-Chao;Song Jing-Yuan
喜树产生喜树碱（CPT），一种广泛用于治疗肺癌、结肠直肠癌、宫颈癌和卵巢癌的单萜吲哚生物碱（MIA）。其生物合成途径已引起人们的极大关注，但APETALA 2/乙烯反应因子（AP 2/ERF）转录因子（TF）对CPT生物合成的调控仍不清楚。本研究系统分析了C.研究了尖叶木的发生、基因结构、保守基序以及不同组织器官（未成熟树皮、子叶、幼花、未成熟果实、成熟果实、成熟叶、根、上茎和下茎）中的基因表达谱。渐尖的共鉴定出198个AP 2/ERF基因，分为5个相对保守的亚家族，包括AP 2（26个基因）、DREB（61个基因）、ERF（92个基因）、RAV（18个基因）和Soloist（1个基因）。结合不同的C.通过系统发育树分析、与CPT生物合成基因的共表达分析以及CPT生物合成途径关键酶基因启动子序列分析，揭示了8个AP 2/ERF转录因子在喜树组织器官中的表达。acuminata可能参与CPT的合成调控，在茎上部或未成熟树皮中表达量较高。其中，四个基因（CacAP 2/ERF 123、CacAP 2/ERF 125、CacAP 2/ERF 126和CacAP 2/ERF 127）属于ERF-B2亚组;两个基因（CacAP 2/ERF 149和CacAP 2/ERF 152）属于ERF-B3亚组;并且另外两个基因（CacAP 2/ERF 095和CacAP 2/ERF 096）属于DREB-A6亚组。这些结果为进一步研究AP 2/ERF基因的功能以促进喜树碱类化合物的生物合成奠定了基础。渐尖的
Camptotheca acuminata produces camptothecin (CPT), a monoterpene indole alkaloid (MIA) that is widely used in the treatment of lung, colorectal, cervical, and ovarian cancers. Its biosynthesis pathway has attracted significant attention, but the regulation of CPT biosynthesis by the APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors (TFs) remains unclear. In this study, a systematic analysis of the AP2/ERF TFs family in C. acuminata was performed, including phylogeny, gene structure, conserved motifs, and gene expression profiles in different tissues and organs (immature bark, cotyledons, young flower, immature fruit, mature fruit, mature leaf, roots, upper stem, and lower stem) of C. acuminata. A total of 198 AP2/ERF genes were identified and divided into five relatively conserved subfamilies, including AP2 (26 genes), DREB (61 genes), ERF (92 genes), RAV (18 genes), and Soloist (one gene). The combination of gene expression patterns in different C. acuminata tissues and organs, the phylogenetic tree, the co-expression analysis with biosynthetic genes, and the analysis of promoter sequences of key enzymes genes involved in CPT biosynthesis pathways revealed that eight AP2/ERF TFs in C. acuminata might be involved in CPT synthesis regulation, which exhibit relatively high expression levels in the upper stem or immature bark. Among these, four genes (CacAP2/ERF123, CacAP 2/ERF 125, CacAP 2/ERF 126, and CacAP2/ERF 127) belong to the ERF-B2 subgroup; two genes (CacAP2/ERF 149 and CacAP2/ERF 152) belong to the ERF-B3 subgroup; and two more genes (CacAP2/ERF095 and CacAP2/ERF096) belong to the DREB-A6 subgroup. These results provide a foundation for future functional characterization of the AP2/ERF genes to enhance the biosynthesis of CPT compounds of C. acuminata.