The effect of environmental heterogeneity on the fitness of antibiotic resistance mutations in Escherichia coli
The effect of environmental heterogeneity on the fitness of antibiotic resistance mutations in Escherichia coli
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DOI：
10.1007/s10682-019-10027-y
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发表时间：
2020-01-07
影响因子：
1.9
通讯作者：
Wong, Alex
中科院分区：
文献类型：
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作者：
Clarke, Leah;Pelin, Adrian;Wong, Alex
抗生素耐药性（AMR）是普遍存在的，耐药病原体不仅存在于临床，而且存在于整个自然和建筑环境中。然而，目前还不清楚AMR在环境中的持续程度：实验室实验表明，耐药性通常代价高昂，因此应该选择对抗。然而，这些研究可能无法推广到更广泛的环境背景。因此，我们估计了大肠杆菌的六个AMR突变体在10种介质中的适应性，反映了生态位，人类宿主和标准实验室条件。我们发现了基因型与环境相互作用的广泛证据，不同的基因型在不同的环境中受到青睐。在某些环境中，抗性等位基因并没有付出代价，这就增加了抗性环境库的可能性。有趣的是，在质量较低的环境中，适应性往往更高。我们的研究结果强调了在AMR管理中需要考虑更广泛环境中的耐药性生态学和进化。
Antimicrobial resistance (AMR) is widespread, with resistant pathogens found not only in the clinic but throughout the natural and built environments. It is unclear, however, to what extent AMR should be expected to persist in the environment: Laboratory experiments have suggested that resistance is typically costly, and hence should be selected against. However, such studies may not generalize to broader environmental settings. As such, we estimated the fitness of six AMR mutants of Escherichia coli in ten media reflecting ecological niches, human hosts, and standard lab conditions. We found extensive evidence for genotype x environment interactions, with different genotypes favored in different environments. Resistance alleles did not suffer a cost in some environments, raising the possibility of environmental reservoirs of resistance. Interestingly, fitness tended to be higher in lower quality environments. Our findings emphasize the need to consider the ecology and evolution of resistance in the broader environment in the management of AMR.