C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature
C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature
复制标题
DOI：
10.1016/j.ab.2011.08.011
复制
发表时间：
2011-12-15
影响因子：
2.9
通讯作者：
Kim, Hie-Joon
中科院分区：
文献类型：
--
作者：
Kim, Jong-Seo;Shin, Mansup;Kim, Hie-Joon
由于C-末端和侧链羧基的化学性质几乎相同，选择性C-末端的衍生化反应一直很困难。恶唑酮C-末端衍生化是目前唯一的选择性C-末端修饰方法，但由于其衍生效率较低，目前还没有得到广泛的应用。本文报道了一种改进的恶唑酮化学方法，用于将BR标记引入到C-末端。溴化肽的MS/MS分析导致了一系列具有BR特征的Y离子，从而促进了从头开始的C-末端测序。(C)2011 Elsevier Inc.保留所有权利。
Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing. (C) 2011 Elsevier Inc. All rights reserved.